Differential regulation ofamoAandamoBgene copies inNitrosomonas europaea
| Autor: | Luis A. Sayavedra-Soto, Lisa Y. Stein, Daniel J. Arp, Norman G. Hommes |
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| Rok vydání: | 2000 |
| Předmět: |
Peptide Biosynthesis
Time Factors Light Operon Protein subunit medicine.disease_cause Microbiology Oxygen Consumption Nitrosomonas europaea Gene expression Genetics medicine Point Mutation Nitrosomonas Molecular Biology Gene Regulation of gene expression Mutation biology Acetylene Gene Expression Regulation Bacterial Ammonia monooxygenase biology.organism_classification Molecular biology Culture Media Quaternary Ammonium Compounds Genes Bacterial Oxidoreductases |
| Zdroj: | FEMS Microbiology Letters. 192:163-168 |
| ISSN: | 1574-6968 0378-1097 |
| Popis: | Nitrosomonas europaea contains two nearly identical copies of the operon, amoCAB, which encodes the ammonia monooxygenase (AMO) enzyme. Cells of N. europaea containing single mutations in either amoA or amoB gene copies were incubated in ammonium both prior to and after exposure to acetylene or light. For each strain, the O(2) consumption rates and amounts of AmoA polypeptide, the active site-containing subunit of AMO, produced in each strain were determined. Strains carrying a mutation in either the amoA(2) or amoB(2) genes responded similarly to wild-type cells, but the strains carrying mutations in the amoA(1) or amoB(1) genes responded differently from the wild-type, or from each other. These results suggest that the copies of amoA and amoB are differentially regulated upon exposure to different external stimuli. |
| Databáze: | OpenAIRE |
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