Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture
Autor: | Margaret S. Inokuma, Choy-Pik Chiu, Michelle A. Waknitz, Melissa K. Carpenter, Michal Amit, James A. Thomson, Joseph Itskovitz-Eldor, Charles P. Harris |
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Rok vydání: | 2000 |
Předmět: |
KOSR
Adult Male Time Factors Rex1 Transplantation Heterologous cloning Cell Culture Techniques Embryoid body Mice SCID Biology Cell Line Mice Animals Humans Cell potency Molecular Biology Telomerase Stem Cells Teratoma Cell Differentiation Cell Biology basic fibroblast growth factor human embryonic stem cells telomeres Embryonic stem cell Molecular biology Clone Cells Culture Media Blastocyst Karyotyping Stem cell Cell Division Adult stem cell Human embryonic stem cell line Developmental Biology Stem Cell Transplantation |
Zdroj: | Developmental biology. 227(2) |
ISSN: | 0012-1606 |
Popis: | Embryonic stem (ES) cell lines derived from human blastocysts have the developmental potential to form derivatives of all three embryonic germ layers even after prolonged culture. Here we describe the clonal derivation of two human ES cell lines, H9.1 and H9.2. At the time of the clonal derivation of the H9.1 and H9.2 ES cell lines, the parental ES cell line, H9, had already been continuously cultured for 6 months. After an additional 8 months of culture, H9.1 and H9.2 ES cell lines continued to: (1) actively proliferate, (2) express high levels of telomerase, and (3) retain normal karyotypes. Telomere lengths, while somewhat variable, were maintained between 8 and 12 kb in high-passage H9.1 and H9.2 cells. High-passage H9.1 and H9.2 cells both formed teratomas in SCID-beige mice that included differentiated derivatives of all three embryonic germ layers. These results demonstrate the pluripotency of single human ES cells, the maintenance of pluripotency during an extended period of culture, and the long-term self-renewing properties of cultured human ES cells. The remarkable developmental potential, proliferative capacity, and karyotypic stability of human ES cells distinguish them from adult cells. |
Databáze: | OpenAIRE |
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