Effect of ultraviolet B (302 nm) irradiation on viability, metabolic and detoxification functions of goat hepatocytes – in vitro study
| Autor: | Syed Muzeeb, Vijayalakshmi Venkateshan, Naseem Begum Shakeel, Mohammed Aejaz Habeeb, C M Habibullah, Aleem Ahmed Khan, Parveen, N. V. S. Rao Mamidi, Ansar Ali Khan, Adarsh K. Capoor |
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| Rok vydání: | 2005 |
| Předmět: |
Cell Survival
Ultraviolet Rays Clinical chemistry Xenotransplantation medicine.medical_treatment Transplantation Heterologous Clinical Biochemistry In Vitro Techniques Biology Lipid peroxidation chemistry.chemical_compound Cytochrome P-450 Enzyme System Bromide Lactate dehydrogenase medicine Animals Irradiation Molecular Biology L-Lactate Dehydrogenase Goats Dose-Response Relationship Radiation Cell Biology General Medicine Metabolism In vitro chemistry Biochemistry Inactivation Metabolic Hepatocytes Lipid Peroxidation |
| Zdroj: | Molecular and Cellular Biochemistry. 277:49-53 |
| ISSN: | 1573-4919 0300-8177 |
| Popis: | The object of the present study was to investigate the effect(s) of UV-B irradiation on the functional integrity, metabolic and detoxifying capacity of the isolated goat hepatocytes. Isolated goat hepatocytes were subjected to UV-B irradiation invitro for 0, 250, 500, 1250, 2500 and 7500 Joules/m2 which correspond to the irradiation time of 0, 1, 2, 5, 10 and 30 min. Cells were then analysed for Viability (Trypan blue exclusion test [TBE], 3-[4,5-dimethylthiozol-2yl]-2,5-diphenyltetrazolium bromide [MTT] assay, Membrane integrity (Lactate dehydrogenase [LDH] leakage, Lipid peroxidation) Detoxification (Ureagenesis, Cytochrome P450 activity [CYP450, Diazepam metabolism] and Glutathione-S-Transferase [GST] activity. The results show that there was no difference in functional, metabolic as well as detoxifying parameters of the hepatocytes when irradiated from 0-1250 Joules/m2, whereas a significant alteration was appreciable in the parameters such as LDH leakage, lipid peroxidation, and CYP450 activity when irradiated beyond 1250 Joules/m2. Our present findings suggest that the biologically compatible and feasible dose of UV-B irradiation for xenotransplantation appears to be 1250 Joules/m2. |
| Databáze: | OpenAIRE |
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