High Azole Resistance in Aspergillus fumigatus Isolates from Strawberry Fields, China, 2018

Autor: Li Han, Chunping Qin, Hong Fan, Yong Chen, Fengshou Dong, Paul E. Verweij, Runan Li, Jingya Zhao, Yongquan Zheng
Rok vydání: 2020
Předmět:
Genotyping Techniques
Epidemiology
Antifungal drug
lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4]
lcsh:Medicine
Asexual sporulation
Aspergillus fumigatus
chemistry.chemical_compound
0302 clinical medicine
short tandem repeat typing
resistance hotspot
Genotype
cyp51A
STR type
030212 general & internal medicine
Phylogeny
Soil Microbiology
agriculture
chemistry.chemical_classification
azole fungicides
strawberry fields
asexual reproduction
biology
antifungal resistance
farms
Crop Production
Fungicide
strawberries
Infectious Diseases
High Azole Resistance in Aspergillus fumigatus Isolates from Strawberry Fields
China
2018
strawberry
Microbiology (medical)
China
030231 tropical medicine
Microbial Sensitivity Tests
Fragaria
lcsh:Infectious and parasitic diseases
Microbiology
03 medical and health sciences
Antibiotic resistance
All institutes and research themes of the Radboud University Medical Center
azole resistance
Drug Resistance
Fungal
lcsh:RC109-216
asexual sporulation
antimicrobial resistance
Tebuconazole
Research
lcsh:R
antifungal drug resistance
MAT1-1
Sequence Analysis
DNA
biology.organism_classification
Fungicides
Industrial
Cross-Sectional Studies
chemistry
Azole
fungi
Microsatellite Repeats
Zdroj: Emerging Infectious Diseases, 26, 81-89
Emerging Infectious Diseases, 26, 1, pp. 81-89
Emerging Infectious Diseases, Vol 26, Iss 1, Pp 81-89 (2020)
Emerging Infectious Diseases
ISSN: 1080-6040
Popis: In 2018, we conducted a cross-sectional study to investigate azole resistance in environmental Aspergillus fumigatus isolates obtained from different agricultural fields in China. Using 63 soil cores, we cultured for azole-resistant A. fumigatus and characterized isolates by their cyp51A gene type, short tandem repeat genotype, and mating type. Of 206 A. fumigatus isolates, 21 (10.2%) were azole resistant. Nineteen of 21 had mutations in their cyp51A gene (5 TR34/L98H, 8 TR34/L98H/S297T/F495I, 6 TR46/Y121F/T289A). Eighteen were cultured from soil samples acquired from strawberry fields, suggesting this soil type is a potential hotspot for azole resistance selection. Twenty resistant isolates were mating type MAT1-1, suggesting asexual sporulation contributed to their evolution. Prochloraz, difenoconazole, and tebuconazole were the most frequently detected fungicides in soil samples with azole-resistant fungus. Our study results suggest that managing the fungicides used in agriculture will help contain the problem of antifungal drug resistance in clinics.
Databáze: OpenAIRE
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