Molecular and Clinical Characteristics of Clonal Complex 59 Methicillin-Resistant Staphylococcus aureus Infections in Mainland China

Autor: X. Shen, Margaret Ip, Chuanqing Wang, Yonghong Yang, Sangjie Yu, Zhou Fu, Yuejie Zheng, Changan Zhao, Mingjiao Sun, Kaihu Yao, Yunxiao Shang, Juan Li, Changcong Li, Li Deng, Guoying Huang, Lijuan Wang, Jing Sun
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Health Screening
Epidemiology
lcsh:Medicine
Pathogenesis
medicine.disease_cause
Pediatrics
Pathology
Prevalence
Prospective Studies
lcsh:Science
Child
Staphylococci
Multidisciplinary
Virulence
Reverse Transcriptase Polymerase Chain Reaction
Microbial Mutation
Child Health
Drug Resistance
Microbial
Staphylococcal Infections
Bacterial Pathogens
Electrophoresis
Gel
Pulsed-Field
Staphylococcus aureus
Medical Microbiology
Medicine
Infectious diseases
Public Health
Molecular Pathology
Research Article
Plasmids
DNA
Bacterial
Methicillin-Resistant Staphylococcus aureus
Pediatric Critical Care
China
Virulence Factors
Bacterial diseases
Microbial Sensitivity Tests
Biology
Staphylococcal infections
Microbiology
Species Specificity
Diagnostic Medicine
Pulsed-field gel electrophoresis
medicine
Humans
Typing
SCCmec
lcsh:R
Bacteriology
Gene Expression Regulation
Bacterial
medicine.disease
Methicillin-resistant Staphylococcus aureus
Virology
Genes
Bacterial
Multilocus sequence typing
lcsh:Q
General Pathology
Multilocus Sequence Typing
Zdroj: PLoS ONE
PLoS ONE, Vol 8, Iss 8, p e70602 (2013)
ISSN: 1932-6203
Popis: Detailed molecular analyses of Clonal Complex 59 (CC59) methicillin-resistant Staphylococcus aureus (MRSA) isolates from children in seven major cities across Mainland China were examined. A total of 110 CC59 isolates from invasive and non-invasive diseases were analyzed by multilocus sequence typing (MLST), Staphylococcus cassette chromosome mec (SCCmec) typing, staphylococcal protein A (spa) typing and pulsed-field gel electrophoresis (PFGE). Antibiotics susceptibilities, carriage of plasmids and 42 virulence genes and the expression of virulence factors were examined. ST59 (101/110, 91.8%) was the predominant sequence type (ST), while single locus variants (SLVs) belonging to ST338 (8/110, 7.3%) and ST375 (1/110, 0.9%) were obtained. Three SCCmec types were found, namely type III (2.7%), type IV (74.5%) and type V (22.7%). Seven spa types including t437, which accounted for 87.3%, were determined. Thirteen PFGE types were obtained. PFGE types A and B were the major types totally accounting for 81.8%. The dominant clone was ST59-t437-IVa (65.5%), followed by ST59-t437-V (14.5%). The positive rate of luks-PV and lukF-PV PVL encoding (pvl) gene was 55.5%. Plasmids were detected in 83.6% (92/110) of the strains. The plasmid size ranging from 23.4 kb to 50 kb was most prevalent which accounted for 83.7% (77/92). A significantly lower expression of hla was found in ST59-t437-IVa compared with ST59-t437-V. Among the 110 cases, 61.8% of the patients were less than 1 year old. A total of 90 cases (81.8%) were community-associated (CA) infections whereas 20 cases (18.2%) were hospital-associated (HA) infections. Out of the 110 patients, 36.4% (40/110) were diagnosed with invasive infectious diseases in which ST59-t437-IVa accounted for 67.5% (27/40). In brief, ST59-t437-IVa was proved as the dominant clone in CC59 MRSA strains. The carriage rate of pvl gene was high. CC59 MRSA could result in CA and HA infections. The majortiy of MRSA infection children were in young age.
Databáze: OpenAIRE
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