Transcutaneous Measurement of Bilirubin in Newborns: Comparison with an Automated Jendrassik–Grof Procedure and HPLC
| Autor: | Steven C. Kazmierczak, Kimberly P. Briley, Alex F. Robertson, Bill L. Kreamer, Glenn R. Gourley |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Standard of care Bilirubin Point-of-Care Systems Clinical Biochemistry Gestational Age High-performance liquid chromatography chemistry.chemical_compound medicine Birth Weight Humans Spectral analysis Chromatography High Pressure Liquid Blood Specimen Collection business.industry Biochemistry (medical) Age Factors Infant Newborn Albumin Jaundice Surgery chemistry Anesthesia Skin color Hemoglobin medicine.symptom business Blood Chemical Analysis |
| Zdroj: | Clinical Chemistry. 50:433-435 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1373/clinchem.2003.027326 |
| Popis: | Measurement of bilirubin in sera of newborn infants is one of the most frequently performed laboratory tests in this patient population (1)(2)(3)(4). Chemical methods for measurement of bilirubin are the standard of care in the assessment of neonatal jaundice. However, transcutaneous techniques for assessing in vivo bilirubin concentrations have been advocated as a more cost-effective and less traumatic alternative to the collection of blood by heel puncture (2)(3). Studies have varied in their assessment of the accuracy of transcutaneous bilirubin assessment; poor to excellent agreement between transcutaneous bilirubin measurements and bilirubin measured in plasma has been reported (1)(2)(3)(5)(6)(7)(8)(9). The best agreement between transcutaneous bilirubin measurements and measurement of plasma bilirubin concentrations has been reported for studies using homogeneous populations of newborns with comparable basal skin color (9). Unfortunately, many factors, such as hemoglobin concentration, the pH and bilirubin-binding capacity of albumin, the technique used to obtain the blood sample, skin pigmentation, and requirement by some methods for baseline measurement of skin color immediately after birth, can significantly effect the agreement between transcutaneous and chemical methods for bilirubin (6)(10)(11). We evaluated the BiliCheck point-of-care device (Respironics, Marietta, GA), which performs transcutaneous measurement of bilirubin by multiwavelength spectral analysis. We compared results obtained with the BiliCheck with bilirubin concentrations in blood specimens measured in a hospital laboratory. In addition, we also measured bilirubin by HPLC. Although HPLC is labor-intensive and not practical for routine use, this method is not subject to interference from hemoglobin or lipemia. Our goals were to evaluate the accuracy of the BiliCheck method compared with a laboratory-based bilirubin method, using HPLC as the comparison method. In addition, we assessed the … |
| Databáze: | OpenAIRE |
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