Molecular detection and occurrence of 'Candidatus Mycoplasma haemobos' in dairy cattle of Southern Brazil
| Autor: | Gislaine Cristina Ferreira da Silva, Odilon Vidotto, Amanda Fonseca Zangirolamo, Laurival Antônio Vilas Boas, Arnaldo Sotero Luz e Souza, Alexey Leon Gomel Bogado, Alexander Welker Biondo, Aline Girotto, João Luis Garcia |
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| Rok vydání: | 2012 |
| Předmět: |
Veterinary medicine
Jersey cattle General Veterinary Candidatus Mycoplasma haemobos business.industry Animal production Biology 16S ribosomal RNA Polymerase Chain Reaction law.invention Dairying Mycoplasma law GenBank Animals Parasitology Livestock Cattle Female business Polymerase chain reaction Dairy cattle Brazil |
| Zdroj: | Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria. 21(3) |
| ISSN: | 1984-2961 |
| Popis: | Bovine hemoplasmas are bacteria found on the erythrocyte surface or free in the plasma of cattle. The aim of the present study was to evaluate the occurrence of 'Candidatus Mycoplasma haemobos' ('C. M. haemobos') in Holstein and Jersey cattle raised in Londrina and surroundings, northern region of the State of Parana, Southern Brazil. PCR testing directed to 16S rRNA gene fragment was performed to investigate the occurrence and characterize the molecular identity of 'C. M. haemobos'. A total of 264/433 (60.97%) blood samples were positive by PCR. Further alignment of 500-bp amplicons to available sequences at the GenBank database showed high identity (100%) to 'C. M. haemobos'. To the author's knowledge, this is the first molecular confirmation of the hemoplasma 'C. M. haemobos' in cattle from Brazil. Moreover, 'C. M. haemobos' was observed in high occurrence in dairy cattle, and may have significant impact in livestock production. |
| Databáze: | OpenAIRE |
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