Expression of RON in colorectal cancer and its relationships with tumor cell behavior and prognosis
| Autor: | Young-Lan Park, Gi-Hoon Lee, Kyu-Yeol Kim, Eun Myung, Jong-Sun Kim, Dae-Seong Myung, Kang-Jin Park, Sung-Bum Cho, Wan-Sik Lee, Young-Do Jung, Hyun-Soo Kim, Young-Eun Joo |
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| Rok vydání: | 2012 |
| Předmět: |
0301 basic medicine
Male Cancer Research Colon 030106 microbiology Kaplan-Meier Estimate 03 medical and health sciences Predictive Value of Tests Humans Neoplasm Invasiveness beta Catenin Aged Receptor Protein-Tyrosine Kinases General Medicine Middle Aged Prognosis Immunohistochemistry Up-Regulation Gene Expression Regulation Neoplastic 030104 developmental biology Oncology Lymphatic Metastasis Female Lymph Nodes Mitogen-Activated Protein Kinases Colorectal Neoplasms Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Tumori. 98(5) |
| ISSN: | 2038-2529 |
| Popis: | Aims and background The aims of the current study were to evaluate whether recepteur d'origine nantais (RON) affects tumor cell behavior and oncogenic signaling pathways in colorectal cancer, and to examine the relationship of its expression with various clinicopathological parameters and patient survival. Methods Immunohistochemistry, Western blot and RT-PCR were used to detect the expression of the RON gene in human colorectal cancer tissue. To study the biological role of RON in tumor cell behavior and cellular signaling pathways, we used small interfering RNA (siRNA) to knock down RON gene expression in human colorectal cancer cell lines. Results Knockdown of RON inhibited the induction of the invasive growth phenotype and the activation of oncogenic signaling pathways including Akt, MAPK and β-catenin. RON overexpression was associated with tumor size, lymphovascular invasion, depth of invasion, lymph node metastasis, distant metastasis, tumor stage and poor survival. Conclusions These results suggest that RON overexpression may help in predicting poor clinical outcomes in colorectal cancer. |
| Databáze: | OpenAIRE |
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