Characterisation of Mycoplasma Pneumoniae Growth Factors In Bovine Serum Fraction
| Autor: | D. C. Thomas, J. H. Hughes, N. L. Somerson, V. V. Hamparian |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Microbiology (medical)
Mycoplasma pneumoniae Fraction (chemistry) medicine.disease_cause Microbiology Mycoplasma medicine Animals High activity Bovine serum albumin Growth Substances Polyacrylamide gel electrophoresis Gel electrophoresis Chromatography biology Temperature Low activity General Medicine Culture Media Molecular Weight Sephadex biology.protein Cattle Electrophoresis Polyacrylamide Gel |
| Zdroj: | Journal of Medical Microbiology. 7:35-40 |
| ISSN: | 1473-5644 0022-2615 |
| Popis: | SUMMARY Commercially available batches of bovine serum fraction (BSF) vary in their ability to promote the growth of Mycoplasma pneumoniae on glass. Poly-acrylamide gel electrophoresis shows the presence in BSF of three major components, which can also be separated by Sephadex chromatography. The slow moving component (SMC) in the gel appears to be the factor responsible for the adherence of M. pneumoniae to glass and its growth on that site; batches of BSF containing 6·15% of SMC possess high activity, those with less than 5% have low activity. Polyacrylamide gel electrophoresis is a simple and precise technique for determining growth-promoting activity of BSF. |
| Databáze: | OpenAIRE |
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