Oxidative DNA damage in human peripheral leukocytes induced by massive aerobic exercise
| Autor: | Kelvin K C Tsai, Kuang Ming Hsu, Tsung-Yun Liu, Chi Woon Kong, Chen Fu Hsu, Tai Ger Hsu, Hu Cheng |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male medicine.medical_specialty DNA Repair Free Radicals DNA repair DNA damage Biochemistry Running Lipid peroxidation chemistry.chemical_compound Physiology (medical) Internal medicine Leukocytes medicine Humans Deoxyguanosine Aerobic exercise Creatine Kinase Exercise Nitrites chemistry.chemical_classification Reactive oxygen species Nitrates Chemistry 8-Hydroxy-2'-deoxyguanosine Molecular biology Aerobiosis Comet assay Kinetics Endocrinology 8-Hydroxy-2'-Deoxyguanosine Lipid Peroxidation Oxidation-Reduction Biomarkers DNA Damage |
| Zdroj: | Free Radical Biology and Medicine. 31:1465-1472 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/s0891-5849(01)00729-8 |
| Popis: | Reactive oxygen species produced during vigorous exercise may permeate into cell nuclei and induce oxidative DNA damage, but the supporting evidence is still lacking. By using a 42 km marathon race as a model of massive aerobic exercise, we demonstrated a significant degree of unrepaired DNA base oxidation in peripheral immunocompetent cells, despite a concurrent increase in the urinary excretion of 8-hydroxy-2'-deoxyguanosine. Single cell gel electrophoresis with the incorporation of lesion-specific endonucleases further revealed that oxidized pyrimidines (endonuclease III-sensitive sites) contributed to most of the postexercise nucleotide oxidation. The oxidative DNA damage correlated significantly with plasma levels of creatinine kinase and lipid peroxidation metabolites, and lasted for more than 1 week following the race. This phenomenon may be one of the mechanisms behind the immune dysfunctions after exhaustive exercise. |
| Databáze: | OpenAIRE |
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