Optimization of penicillin G acylase immobilized on glutaraldehyde-modified titanium dioxide
| Autor: | Yongshan Zhou, Zhangjun Chen, Runtian Wang, Zhenbin Chen, Chunli Liu, Xudong Wang |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Biomedical Engineering Bioengineering 01 natural sciences Applied Microbiology and Biotechnology Catalysis 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Potassium penicillin g Drug Discovery Thermal stability 030304 developmental biology Titanium 0303 health sciences Process Chemistry and Technology Temperature Substrate (chemistry) General Medicine Hydrogen-Ion Concentration Enzymes Immobilized Solutions Penicillin G Acylase chemistry Glutaral Titanium dioxide Molecular Medicine Glutaraldehyde Penicillin Amidase Biotechnology Nuclear chemistry |
| Zdroj: | Biotechnology and applied biochemistry6. References. 66(6) |
| ISSN: | 1470-8744 |
| Popis: | In this work, TiO2 , which was modified by glutaraldehyde, was adopted as the carrier; the penicillin G acylase (PGA) was immobilized and the influence of immobilized conditions, such as pH of solution, the concentration of PGA, the immobilization temperature, and the reaction time, on the catalytic performance of the immobilized PGA was investigated and optimized. During this process, potassium penicillin G (PG) was chosen as substrate, and the quantity of 6-aminopenicillanic acid (6-APA) produced by PG at the temperature of 25 °C for 3 Min in neutral solution was conscripted as the evaluation foundation, indexes, containing the loading capacity (ELC), the activity (EA), and activity retention rate (EAR), were calculated based on quantities of produced 6-APA and compared with finding out the suitable conditions. Results showed that when the solution pH, PGA concentration, immobilization temperature, and reaction time were 8.0, 2.5% (v/v), 35 °C, and 24 H, respectively, ELC, EA, and EAR presented optimal values of 9,190 U, 14,969 U/g, and 88.5% relatedly. After that, the stability and reusability of immobilized PGA were studied, and the results documented that the pH resistance, thermal stability, and storage stability of immobilized PGA were significantly improved. This work provided technique support for the practical application of immobilized PGA carrier. |
| Databáze: | OpenAIRE |
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