Self-tuning interfacial architecture for Estradiol detection by surface plasmon resonance biosensor
Autor: | Borys Snopok, Svitlana Shinkaruk, Luc Vellutini, Praskoviya Boltovets |
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Rok vydání: | 2017 |
Předmět: |
Stretched exponential function
Imagination media_common.quotation_subject Biomedical Engineering Biophysics Nanotechnology Biosensing Techniques 02 engineering and technology 01 natural sciences Limit of Detection Electrochemistry Electrostatic levitation Surface plasmon resonance media_common Estradiol Chemistry 010401 analytical chemistry Serum Albumin Bovine General Medicine Surface Plasmon Resonance 021001 nanoscience & nanotechnology Electrostatics 0104 chemical sciences Chemical physics Gold 0210 nano-technology Biosensor Biotechnology Macromolecule Conjugate |
Zdroj: | Biosensors and Bioelectronics. 90:91-95 |
ISSN: | 0956-5663 |
Popis: | This study reports the operation principles for reusable SPR biosensors utilizing nanoscale-specific electrostatic levitation phenomena in their sensitive layer design. Functional macromolecular building blocks localized near the "charged" surface by a variety of weak electrostatic interactions create a flexible and structurally variable architecture. A proof-of-concept is demonstrated by an immunospecific detection of 17β-Estradiol (E2) following the competitive inhibition format. The sensing interfacial architecture is based on the BSA-E2 conjugate within the BSA matrix immobilized on the "charged" (as a result of guanidine thiocyanate treatment) gold surface at pH 5.0. Kinetic analysis for different E2 concentrations shows that using parameter β of the stretched exponential function ~(1-exp(-(t/τ)β) as an analyte-specific response measure allows one to substantially decrease the low detection limit (down to 10-3ng/ml) and increase the dynamic range (10-3-103ng/ml) of the SPR biosensor. Finally, it's concluded that the created interfacial architecture is a typical complex system, where SPR response is formed by the stochastic interactions within the whole variety of processes in the system. The E2 addition destroys the uniformity of the reaction space (where an interaction of the antibody (Ab) and the analog of E2 in the self-tuneable matrix takes place) by the redistribution of the immunospecific complexes Ab(E2)x (x=0, 1, 2) dependent on E2 concentration. Binding dynamics changes are reflected in the values of β which summarize in compact form all "hidden" information specific for the evolving distributed interfacial system. |
Databáze: | OpenAIRE |
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