Expression and tumor-promoting effects of caprin-1 in human glioma
| Autor: | Xiang-Jun Tang, Li Zhang, Hui Gui, Lu Junti, Bin Wang, Long-Jun Dai, Zhuo-Shun Yang, Yan Lidong, Dan-Dan Zou, Jie Luo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Caprin-1
Small interfering RNA medicine.diagnostic_test Cell growth proliferation Cell migration Transfection Biology Immunofluorescence medicine.disease migration lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens lcsh:RC254-282 gliomas 03 medical and health sciences 0302 clinical medicine Cytoplasm 030220 oncology & carcinogenesis Glioma Immunoblot Analysis medicine Cancer research PI3K/AKT/mTOR pathway 030217 neurology & neurosurgery |
| Zdroj: | Glioma, Vol 1, Iss 4, Pp 136-141 (2018) |
| ISSN: | 2589-6121 2589-6113 |
| Popis: | Background: Cytoplasmic activation/proliferation-associated protein-1 (caprin-1) is a newly discovered RNA-binding protein and is now recognized as one of the putative oncogenes. This study was performed to reveal its presence in human gliomas and its oncogenic functions in human glioblastoma-derived Denver brain tumor research group 05 (DBTRG-05MG) cells. Materials and Methods: Clinical glioma samples were cumulatively collected for the identification of caprin-1 using immunoblot analysis and immunofluorescence detection. DBTRG cells transfected with caprin-1-specific small interfering RNA (siRNA) were used to verify caprin-1's oncogenic function using a real-time cell analyzer (RTCA) and scratch assay. Results: Seven of eight collected glioma samples were identified as positive for caprin-1 expression. siRNA dose-responsive inhibition of cell proliferation was observed in DBTRG cells with RTCA, and cell migration rate was significantly reduced by siRNA transfection (P < 0.05). Conclusion: The present study identified the higher expression of caprin-1 in human glioblastoma-derived DBTRG cells. Its oncogenic functions, mainly enhanced cell proliferation and promoted cell migration capacity, were also verified in these cells. This study provided fundamentals for developing caprin-1 as a therapeutic target for the treatment of gliomas. |
| Databáze: | OpenAIRE |
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