Regulation of angiogenic factors in angiotensin II infusion model in association with tubulointerstitial injuries
| Autor: | Yuki Takazawa, Hirofumi Makino, Yoshihiko Yamamoto, Hitoshi Sugiyama, Yohei Maeshima, Kumiko Hirokoshi, Yasushi Yamasaki, Kunihiro Ichinose, Yan Wu, Hiroyuki Kitayama |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty Hypertension Renal medicine.drug_class Vascular permeability Blood Pressure Peritubular capillaries Monocytes Angiopoietin-2 Rats Sprague-Dawley chemistry.chemical_compound Internal medicine Internal Medicine medicine Angiopoietin-1 Animals Infusions Parenteral Receptor business.industry Angiotensin II Macrophages Hydralazine Receptor antagonist Receptor TIE-2 Rats Vascular endothelial growth factor Disease Models Animal Proteinuria medicine.anatomical_structure Endocrinology Losartan Kidney Tubules chemistry cardiovascular system Kidney Diseases business hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | American journal of hypertension. 19(7) |
| ISSN: | 0895-7061 |
| Popis: | Background Among various angiogenic factors, vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang1), and angiopoietin-2 (Ang2) play crucial roles in regulating angiogenesis and vascular integrity. Infusion of angiotensin-II (ang II) induces hypertension and focal renal tubulointerstitial injuries. In the present study we investigated the renal expression of VEGF, Ang1, Ang2, and corresponding receptors in association with tubulointerstitial lesions in a rat ang II infusion model. Methods Male Sprague-Dawley (SD) rats received an infusion of ang II or norepinephrine (NE) through osmotic minipumps for 14 days. Angiotensin II type 1 (AT 1 ) or type 2 (AT 2 ) receptor antagonist (losartan or PD123319, respectively) or hydralazine was co-administered. Results Interstitial fibrosis, infiltration of monocyte/macrophage, and peritubular capillary rarefaction induced by ang II was significantly attenuated in the losartan- or PD123319-treated groups. Immunoreactivity of VEGF and Ang1 in cortical tubules was increased by ang II and was attenuated by losartan or PD123319. The increase of VEGF induced by ang II was suppressed by losartan, and the increase of Ang1 induced by ang II was inhibited by PD123319 as detected by immunoblot. The increase of flk-1 and flt-1 (VEGF receptors) and tie-2 (Ang1 receptor) induced by ang II was significantly suppressed by PD123319. These alterations were not observed in hydralazine plus ang II or NE-infused animals. Conclusions These results demonstrate that an infusion of ang II induced the expression of VEGF mainly through AT 1 receptors, and increased the expression of VEGF receptors, tie-2, and Ang1/Ang2 ratio mainly through AT 2 receptors. The increase of VEGF/flk-1/flt-1 may be associated with vascular permeability, monocyte/macrophage infiltration, and rarefaction of peritubular capillaries, and the increase of the Ang1/Ang2 ratio may be a compensatory mechanism counteracting the permeability inducing effect of VEGF after ang II infusion. |
| Databáze: | OpenAIRE |
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