Stromal expression of hemopexin is associated with lymph-node metastasis in pancreatic ductal adenocarcinoma
Autor: | Tatsuo Hata, Hiroki Shima, Takanori Morikawa, Masahiro Iseki, Takeshi Naitoh, Tomoyoshi Tachibana, Masamichi Mizuma, Hiroki Hayashi, Michiaki Unno, Fuyuhiko Motoi, Kazuhiko Igarashi, Kei Nakagawa, Yukie Suzuki, Kei Kawaguchi, Mitsuhiro Shimura, Takashi Suzuki, Takeshi Aoki, Tatsuyuki Takadate |
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Rok vydání: | 2020 |
Předmět: |
Proteomics
Male 0301 basic medicine Proteome Lymphovascular invasion Protein Expression Biochemistry Metastasis 0302 clinical medicine Cell Movement Hemopexin Tandem Mass Spectrometry Basic Cancer Research Medicine and Health Sciences Lymph node Chromatography High Pressure Liquid Multidisciplinary Middle Aged Prognosis Immunohistochemistry Cell Motility medicine.anatomical_structure Oncology Lymphatic Metastasis 030220 oncology & carcinogenesis Medicine Female Anatomy Research Article Nutrient and Storage Proteins Carcinoma Pancreatic Ductal Histology Science Motor Proteins Cell Migration Research and Analysis Methods Lymphatic System 03 medical and health sciences Molecular Motors Cell Line Tumor Pancreatic cancer Gene Expression and Vector Techniques Carcinoma medicine Humans Molecular Biology Techniques Molecular Biology Aged Molecular Biology Assays and Analysis Techniques Tumor microenvironment business.industry Biology and Life Sciences Proteins Cancer Cell Biology medicine.disease Pancreatic Neoplasms 030104 developmental biology Apoferritins Cancer research Lymph Nodes Neoplasm Grading business Developmental Biology |
Zdroj: | PLoS ONE, Vol 15, Iss 7, p e0235904 (2020) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | Pancreatic ductal adenocarcinoma is one of the most aggressive types of cancer. Certain proteins in the tumor microenvironment have attracted considerable attention owing to their association with tumor invasion and metastasis. Here, we used proteomics to identify proteins associated with lymph-node metastasis, which is one of the prognostic factors. We selected lymph node metastasis-positive and -negative patients (n = 5 each) who underwent pancreatectomy between 2005 and 2015 and subjected to comprehensive proteomic profiling of tumor stroma. A total of 490 proteins were detected by mass spectrometry. Software analysis revealed that nine of these proteins were differentially expressed between the two patient groups. We focused on hemopexin and ferritin light chain based on immunohistochemistry results. We assessed the clinicopathological data of 163 patients and found that hemopexin expression was associated with UICC N2 (p = 0.0399), lymph node ratio (p = 0.0252), venous invasion (p = 0.0096), and lymphatic invasion (p = 0.0232). Notably, in vitro assays showed that hemopexin promotes invasion of the pancreatic cancer cells. Our findings suggest that hemopexin is a lymph node metastasis-associated protein that could potentially serve as a useful therapeutic target or biomarker of pancreatic ductal adenocarcinoma. |
Databáze: | OpenAIRE |
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