Stromal expression of hemopexin is associated with lymph-node metastasis in pancreatic ductal adenocarcinoma

Autor: Tatsuo Hata, Hiroki Shima, Takanori Morikawa, Masahiro Iseki, Takeshi Naitoh, Tomoyoshi Tachibana, Masamichi Mizuma, Hiroki Hayashi, Michiaki Unno, Fuyuhiko Motoi, Kazuhiko Igarashi, Kei Nakagawa, Yukie Suzuki, Kei Kawaguchi, Mitsuhiro Shimura, Takashi Suzuki, Takeshi Aoki, Tatsuyuki Takadate
Rok vydání: 2020
Předmět:
Proteomics
Male
0301 basic medicine
Proteome
Lymphovascular invasion
Protein Expression
Biochemistry
Metastasis
0302 clinical medicine
Cell Movement
Hemopexin
Tandem Mass Spectrometry
Basic Cancer Research
Medicine and Health Sciences
Lymph node
Chromatography
High Pressure Liquid
Multidisciplinary
Middle Aged
Prognosis
Immunohistochemistry
Cell Motility
medicine.anatomical_structure
Oncology
Lymphatic Metastasis
030220 oncology & carcinogenesis
Medicine
Female
Anatomy
Research Article
Nutrient and Storage Proteins
Carcinoma
Pancreatic Ductal
Histology
Science
Motor Proteins
Cell Migration
Research and Analysis Methods
Lymphatic System
03 medical and health sciences
Molecular Motors
Cell Line
Tumor
Pancreatic cancer
Gene Expression and Vector Techniques
Carcinoma
medicine
Humans
Molecular Biology Techniques
Molecular Biology
Aged
Molecular Biology Assays and Analysis Techniques
Tumor microenvironment
business.industry
Biology and Life Sciences
Proteins
Cancer
Cell Biology
medicine.disease
Pancreatic Neoplasms
030104 developmental biology
Apoferritins
Cancer research
Lymph Nodes
Neoplasm Grading
business
Developmental Biology
Zdroj: PLoS ONE, Vol 15, Iss 7, p e0235904 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Pancreatic ductal adenocarcinoma is one of the most aggressive types of cancer. Certain proteins in the tumor microenvironment have attracted considerable attention owing to their association with tumor invasion and metastasis. Here, we used proteomics to identify proteins associated with lymph-node metastasis, which is one of the prognostic factors. We selected lymph node metastasis-positive and -negative patients (n = 5 each) who underwent pancreatectomy between 2005 and 2015 and subjected to comprehensive proteomic profiling of tumor stroma. A total of 490 proteins were detected by mass spectrometry. Software analysis revealed that nine of these proteins were differentially expressed between the two patient groups. We focused on hemopexin and ferritin light chain based on immunohistochemistry results. We assessed the clinicopathological data of 163 patients and found that hemopexin expression was associated with UICC N2 (p = 0.0399), lymph node ratio (p = 0.0252), venous invasion (p = 0.0096), and lymphatic invasion (p = 0.0232). Notably, in vitro assays showed that hemopexin promotes invasion of the pancreatic cancer cells. Our findings suggest that hemopexin is a lymph node metastasis-associated protein that could potentially serve as a useful therapeutic target or biomarker of pancreatic ductal adenocarcinoma.
Databáze: OpenAIRE
Externí odkaz: