Physiological roles of endogenous ouabain in normal rats
| Autor: | Haim Rosen, Moran Dvela, Vincent U. Igbokwe, Maoz Nesher, David Lichtstein |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Physiology Natriuresis Aorta Thoracic Blood Pressure Vasodilation Kidney Weight Gain Antibodies Ouabain Phenylephrine Atrial natriuretic peptide Physiology (medical) Internal medicine medicine Animals Homeostasis Vasoconstrictor Agents Rats Wistar Infusions Intravenous Aldosterone Dose-Response Relationship Drug Adrenal gland Chemistry Sodium Kidney metabolism Saponins Rats Cardenolides medicine.anatomical_structure Endocrinology Vasoconstriction Adrenal Cortex Rabbits Sodium-Potassium-Exchanging ATPase Corticosterone Cardiology and Cardiovascular Medicine Atrial Natriuretic Factor medicine.drug |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 297:H2026-H2034 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.00734.2009 |
| Popis: | Endogenous ouabain (EO)-like compounds are synthesized in and released from the adrenal gland. Although EO has been implicated in several pathological states such as hypertension and heart and kidney failure, its physiological roles in normal animal have not been elucidated. To address this issue, we studied the effects of reduction in plasma EO resulting from antiouabain antibody administration. Normal rats were treated for 28 days with antiouabain antibodies or rabbit IgG as control. Infusions were delivered through a jugular vein cannula by osmotic pumps, and blood pressure was monitored by tail-cuff plethysmography. The animals were housed in metabolic cages to measure water and food consumption and urine excretion. After 28 days, the thoracic aorta was isolated and used to study phenylephrine-induced contraction and atrial natriuretic peptide (ANP)-induced vasorelaxation. The adrenal gland cortex was enlarged in the antiouabain antibody-treated rats. Moreover, on the second day of treatment, there was a significant transient reduction in natriuresis in the antiouabain antibody-treated rats, suggesting that EO is a natriuretic hormone. Reduction in natriuresis was also observed when EO levels were reduced by active immunization resulting from sequential injection of ouabain-albumin. Furthermore, following 28 days of treatment, the response to phenylephrine was significantly lowered and that to ANP was significantly increased in aortic rings from antiouabain antibody-treated rats. These findings show for the first time that circulatory ouabain plausibly originating in the adrenal has physiological roles controlling vasculature tone and sodium homeostasis in normal rats. |
| Databáze: | OpenAIRE |
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