Comparative analysis of genetic variation in kava (Piper methysticum) assessed by SSR and DArT reveals zygotic foundation and clonal diversification
| Autor: | Henri Vandenbroucke, Pierre Mournet, Roger Malapa, Jean-Christophe Glaszmann, Hana Chaïr, Vincent Lebot |
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| Rok vydání: | 2015 |
| Předmět: |
Genetic Markers
DNA Plant Genotype Piper methysticum Sélection Biology F30 - Génétique et amélioration des plantes Evolution Molecular Variation génétique Génétique des populations Botany Genetic variation Genetics Cluster Analysis Marqueur génétique Variété Selection Genetic Molecular Biology Kava Oligonucleotide Array Sequence Analysis computer.programming_language clone Principal Component Analysis Dart Genetic Variation food and beverages F70 - Taxonomie végétale et phytogéographie General Medicine Founder Effect Genetic structure U30 - Méthodes de recherche human activities computer Microsatellite Repeats Biotechnology |
| Zdroj: | Genome (Ottawa) |
| ISSN: | 1480-3321 0831-2796 |
| DOI: | 10.1139/gen-2014-0166 |
| Popis: | Kava (Piper methysticum) is a major cash crop in the Pacific. The aim of this study was to assess genetic variation among 103 accessions of kava using SSRs and DArTs. Genetic structure was determined using clustering analyses (WPGMA) and principal coordinate analyses (PCA). Thirteen SSR primers and 75 DArT markers were found polymorphic, and the two types of markers generated similar clustering patterns. Genetic distances ranged from 0 to 0.65 with an average of 0.24 using SSRs and from 0 to 0.64 with an average of 0.24 using DArT. Eleven genotypes were identified with SSR while 28 genotypes were identified with DArT markers. By combining the two sets of markers, a total of only 30 distinct genotypes were observed. In the Vanuatu archipelago, noble cultivars originating from different islands clustered together within a very narrow genetic base despite their diversity of morphotypes. SSR and DArT fingerprints allowed the identification of kava cultivars unsuitable for consumption, so called two-days, and clearly differentiated the wild types classified as P. methysticum var. wichmannii from the cultivars as var. methysticum. Molecular data reveals that all noble cultivars evolved by the predominance of clonal selection. Although they are represented by clearly distinct morphotypes, these cultivars are genetically vulnerable and their potential to adapt to forthcoming changes is limited. These newly developed markers provide high resolution and will be useful for kava diversity analyses and quality assessment. |
| Databáze: | OpenAIRE |
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