Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements
| Autor: | Marie Pospíšilová, Steven Ripp, Gabriela Kuncová, Josef Trögl, Jakub Zajíc |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Pseudomonas putida TVA8
Optical fiber bioluminescent bioreporter 02 engineering and technology Biosensing Techniques 010501 environmental sciences lcsh:Chemical technology 01 natural sciences Biochemistry Hydrocarbons Aromatic Article Analytical Chemistry law.invention optical fiber biosensor chemistry.chemical_compound Escherichia coli 652T7 law Escherichia coli Bioluminescence toluene lcsh:TP1-1185 Electrical and Electronic Engineering Instrumentation Optical Fibers 0105 earth and related environmental sciences chemistry.chemical_classification Chromatography biology Chemistry Pseudomonas putida whole-cell biosensor Contamination 021001 nanoscience & nanotechnology biology.organism_classification Toluene Atomic and Molecular Physics and Optics Triethoxysilane Luminescent Measurements Bioreporter 0210 nano-technology Aromatic hydrocarbon |
| Zdroj: | Sensors (Basel, Switzerland) Sensors Volume 20 Issue 11 Sensors, Vol 20, Iss 3237, p 3237 (2020) |
| ISSN: | 1424-8220 |
| Popis: | In this study, we show the repetitive detection of toluene on a tapered optical fiber element (OFE) with an attached layer of Pseudomonas putida TVA8 bioluminescent bioreporters. The bioluminescent cell layer was attached on polished quartz modified with (3-aminopropyl)triethoxysilane (APTES). The repeatability of the preparation of the optical probe and its use was demonstrated with five differently shaped OFEs. The intensity of measured bioluminescence was minimally influenced by the OFE shape, possessing transmittances between 1.41% and 5.00%. OFE probes layered with P. putida TVA8 were used to monitor liquid toluene over a two-week period. It was demonstrated that OFE probes layered with positively induced P. putida TVA8 bioreporters were reliable detectors of toluene. A toluene concentration of 26.5 mg/L was detected after < 30 min after immersion of the probe in the toluene solution. Additional experiments also immobilized constitutively bioluminescent cells of E. coli 652T7, on OFEs with polyethyleneimine (PEI). These OFEs were repetitively induced with Lauria-Bertani (LB) nutrient medium. Bioluminescence appeared 15 minutes after immersion of the OFE in LB. A change in pH from 7 to 6 resulted in a decrease in bioluminescence that was not restored following additional nutrient inductions at pH 7. The E. coli 652T7 OFE probe was therefore sensitive to negative influences but could not be repetitively used. |
| Databáze: | OpenAIRE |
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