In vitroβ‐carotene toxicity for human colon cancer cells
| Autor: | Shaukat Iftikhar, Helen Lietz, Thomas O. Frommel, Sohrab Mobarhan |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Vitamin
Cancer Research Cell Survival medicine.medical_treatment Medicine (miscellaneous) Biology chemistry.chemical_compound Tumor Cells Cultured medicine Humans Vitamin E Cytotoxic T cell Furans Cytotoxicity Chromatography High Pressure Liquid Carcinogen Nutrition and Dietetics Carotene beta Carotene Molecular biology In vitro Solubility Oncology chemistry Cell culture Colonic Neoplasms Toxicity Immunology Cell Division |
| Zdroj: | Nutrition and Cancer. 25:221-230 |
| ISSN: | 1532-7914 0163-5581 |
| DOI: | 10.1080/01635589609514446 |
| Popis: | Experiments were conducted to determine the effect of beta-carotene on human colon cancer cells in vitro. beta-Carotene solubilized in tetrahydrofuran (THF) was determined to be cytotoxic for three different cell lines: LS 180, SW 620, and HCT-15. The number of LS 180 and SW 620 cells surviving treatment with 2.9 microM beta-carotene was significantly reduced relative to THF-treated cells, and a similar reduction was achieved in HCT-15 cells with use of 5.8 microM beta-carotene. These concentrations are in the range achieved in serum of individuals supplemented with beta-carotene at 30 mg/day. There was no beta-carotene cytotoxicity in the concentration range that characterizes serum of unsupplemented individuals. Vitamin E at200 microM was not cytotoxic and at higher concentrations slightly stimulated proliferation of all three cell lines. Exposure of cells to vitamin E did not diminish the cytotoxicity of beta-carotene, suggesting that the toxic effect of beta-carotene is not due to prooxidant activity. Percent cytotoxicity was increased by extending the duration of exposure of cells to beta-carotene. Interestingly, beta-carotene cytotoxicity decreased with increasing cell density. This density-dependent toxicity was attributable to a higher beta-carotene concentration per cell for cells plated at lower densities. Thus toxicity of beta-carotene for colon cancer cells is dose, time, and cell density dependent and occurs in vitro at concentrations that can be achieved safely in humans. |
| Databáze: | OpenAIRE |
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