A long non‐coding apple RNA, MSTRG.85814.11, acts as a transcriptional enhancer ofSAUR32and contributes to the Fe‐deficiency response
| Autor: | Ting Wu, Yaqiang Sun, Zhenhai Han, Ji Tian, Xinzhong Zhang, Yi Wang, Xuefeng Xu, Pengbo Hao, Xinmin Lv |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Malus Plant Science Biology 01 natural sciences 03 medical and health sciences Downregulation and upregulation Gene Expression Regulation Plant Auxin Genetics RNA Messenger Enhancer Gene Plant Proteins chemistry.chemical_classification fungi Alternative splicing RNA Iron Deficiencies Cell Biology biology.organism_classification Apoplast Cell biology 030104 developmental biology chemistry RNA Plant RNA Long Noncoding Transcription Factors 010606 plant biology & botany |
| Zdroj: | The Plant Journal. 103:53-67 |
| ISSN: | 1365-313X 0960-7412 |
| Popis: | Iron (Fe) is an essential plant nutrient and its deficiency typically limits plant growth. Long non-coding (lnc) RNAs are involved in adaptive responses to nutrient stress; however, it is not known whether they function in the regulation of the canonical Fe-deficiency response. The expression of Malus domestica (apple) lncRNA MSTRG.85814 is induced by Fe deficiency, as identified by high-throughput strand-specific RNA-seq analysis of an apple homograft system. MSTRG.85814 has a complex structure, with 13 predicted RNA sequence variants, four of which are upregulated in the roots of plants experiencing Fe deficiency. We found that one MSTRG.85814 splice variant (MSTRG.85814.11) positively modulated its cis target mRNA derived from the small auxin upregulated gene SAUR32. This in turn promoted the expression of SAUR32 and caused an increase in the expression of a plasma membrane proton ATPase, AHA10. Using a pH imaging technique, a significant decrease in the apoplastic pH was observed to occur in the root tips of MSTRG.85814.11 or SAUR32-overexpressing apple plants. Thus MSTRG.85814.11 was shown to positively promote SAUR32 expression, which then activated proton extrusion involved in the Fe-deficiency response. These results reveal a mechanism by which lncRNA promotes environmental Fe-deficiency stress adaption. |
| Databáze: | OpenAIRE |
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