Monitoring cell-specific neutral lipid accumulation inPhaeodactylum tricornutum(Bacillariophyceae) with Nile Red staining - a new method for FlowCAM
| Autor: | Jukka Pellinen, Riikka-Juulia Koivula, Katariina Natunen, Jukka Seppälä |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Plant Science Aquatic Science Biology 01 natural sciences chemistry.chemical_compound 010608 biotechnology Oxazines Botany Phytoplankton Phaeodactylum tricornutum Diatoms 010604 marine biology & hydrobiology ta1183 Nile red Spectrofluorometer Lipid Measurement Lipid Metabolism biology.organism_classification Fluorescence Staining Spectrometry Fluorescence chemistry Biochemistry ta1181 Biological Assay lipids (amino acids peptides and proteins) Gas chromatography |
| Zdroj: | Journal of Phycology. 53:396-404 |
| ISSN: | 0022-3646 |
| DOI: | 10.1111/jpy.12504 |
| Popis: | With the fluorescent stain Nile Red (NR), phytoplankton lipid accumulation can be monitored quickly and in situ. In the light of recent results in phytoplankton diversity research, there is also a need for cell- and species-specific lipid measurement techniques. The objective of this work was to investigate whether cell-specific phytoplankton lipid accumulation could be monitored with the image-based particle analyzer FlowCAM™ and NR staining. Applying Phaeodactylum tricornutum as a model species, we compared the FlowCAM method to two established lipid quantification methods: spectrofluorometric NR fluorescence measurement and total lipid analysis by gas chromatography. The experiment was carried out in batch cultures under nitrogen limitation to induce lipid accumulation. We showed significant correlation between the three different lipid quantification methods confirming the applicability of the novel FlowCAM method in cell-specific and near real-time lipid quantification. Furthermore, with the method described here, the lipid content of taxonomically distinguished cells can eventually be measured from multispecies cultures, opening several new possibilities to study species-specific responses to stress conditions and the complementarity effect. |
| Databáze: | OpenAIRE |
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