Inositol 1,4,5-trisphosphate 3-kinase B (Itpkb) controls survival, proliferation and cytokine production in mouse peripheral T cells
| Autor: | Christophe Frippiat, Yoann Maréchal, Stéphane Schurmans, Christophe Erneux, Valérie Pouillon |
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| Rok vydání: | 2012 |
| Předmět: |
CD4-Positive T-Lymphocytes
Cancer Research medicine.medical_specialty Cell Survival Transgene medicine.medical_treatment Cellular differentiation Gene Expression Biology CD8-Positive T-Lymphocytes Mice Internal medicine Genetics medicine Animals Molecular Biology Cells Cultured Cell Proliferation Mice Knockout Thymocytes Cell growth Cell Differentiation T lymphocyte Cell biology Phosphotransferases (Alcohol Group Acceptor) Endocrinology Cytokine Mature Thymocyte T cell differentiation Molecular Medicine Cytokines Immunologic Memory CD8 Signal Transduction |
| Zdroj: | Advances in biological regulation. 53(1) |
| ISSN: | 2212-4934 |
| Popis: | Mice genetically-deficient for the B isoform of the inositol 1,4,5-trisphosphate 3-kinase (or Itpkb) have a severe defect in thymocytes differentiation and thus lack peripheral T cells. In order to study the functional role of Itpkb in peripheral T cells, we constructed a new mouse where a transgene encoding mouse Itpkb is specifically and transiently expressed in thymocytes of Itpkb(-)(/)(-) mice. This allows a partial rescue of mature thymocyte/T cell differentiation and thus the functional characterization of peripheral T cells lacking Itpkb. We show here that Itpkb(-)(/)(-) CD4(+) and CD8(+) peripheral T cells present important functional alterations. Indeed, an increased activated/memory phenotype as well as a decreased proliferative capacity and survival were detected in these T cells. These Itpkb-deficient peripheral T cells have also an increased capacity to secrete cytokines upon stimulation. Together, our present results define the important role of Itpkb in peripheral mature T cell fate and function in mouse, suggesting a potential role for Itpkb in autoimmunity. |
| Databáze: | OpenAIRE |
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