Bone marrow transplantation in miniature swine. I. Development of the model
| Autor: | L. R. Pennington, Thomas J. MacVittie, Kaoru Sakamoto, Margaret A. Mcdonough, David H. Sachs, Ronald E. Gress, Frederique A. Popitz-bergez, Mark D. Pescovitz |
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| Rok vydání: | 1988 |
| Předmět: |
Male
Pathology medicine.medical_specialty Swine medicine.medical_treatment Miniature swine Transplantation Autologous Lymphocyte Depletion Major Histocompatibility Complex Animal model Postoperative Complications Histocompatibility Antigens medicine Animals Transplantation Homologous Autogenous bone Bone Marrow Transplantation Transplantation Marrow transplantation business.industry Graft Survival T lymphocyte Radiation therapy medicine.anatomical_structure Immunology Swine Miniature Female Bone marrow Whole body business Whole-Body Irradiation |
| Zdroj: | Transplantation. 45(1) |
| ISSN: | 0041-1337 |
| Popis: | Procedures for successful autologous and MHC-matched allogeneic bone marrow transplantation in partially inbred, MHC-defined miniature swine have been established. All marrow recipients were conditioned with single-dose total-body irradiation at the upper level of tolerance, and supported with antibiotics and irradiated blood products during aplasia. Surgical harvest of autologous and allogeneic marrow yielded sufficient numbers of cells to successfully reconstitute recipients. Radiation control animals that received no marrow failed to show recovery of marrow function. Pigs transplanted with autologous marrow at doses greater than 10(8) cells/kg routinely engrafted and recovered normal marrow function. The major clinical complications were acute and chronic infections and hemorrhage. T cell-depleted autologous marrow also engrafted, and there was no observed increase in clinical complications. In bone marrow transplantation across non-MHC allogeneic differences, engraftment and survival were similar to that observed for autologous transplants. The T cell depletion of marrow in such MHC-matched allogeneic recipients was associated in one animal, however, with early reconstitution by cells of autologous origin. |
| Databáze: | OpenAIRE |
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