A piglet model of iatrogenic rectosigmoid hypoganglionosis reveals the impact of the enteric nervous system on gut barrier function and microbiota postnatal development
| Autor: | Francis LeGouevec, Juliette Hascoet, Pauline Berneau, Julien Georges, Gaëlle Boudry, Sébastien Henno, Alexis Arnaud, Michèle Formal, Gwenaëlle Randuineau |
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| Přispěvatelé: | Nutrition, Métabolismes et Cancer (NuMeCan), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Service de chirurgie pédiatrique [Rennes] = Paediatric / Pediatric surgery [Rennes], CHU Pontchaillou [Rennes], Physiologie et Phénotypage des Porcs (UE 3P ), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Bernard, Emilie |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty Hirschsprung disease Swine medicine.medical_treatment [SDV]Life Sciences [q-bio] Iatrogenic Disease Intestinal permeability Enteric Nervous System Proinflammatory cytokine 03 medical and health sciences 0302 clinical medicine 030225 pediatrics medicine Animals Saline Myenteric plexus Enterocolitis business.industry Microbiota Tight junction protein General Medicine Hypoganglionosis medicine.disease Pathophysiology [SDV] Life Sciences [q-bio] 030220 oncology & carcinogenesis Models Animal Pediatrics Perinatology and Child Health Female Surgery Enteric nervous system medicine.symptom business |
| Zdroj: | Journal of Pediatric Surgery Journal of Pediatric Surgery, American Academy of Pediatrics, 2021, 56 (2), pp.337-345. ⟨10.1016/j.jpedsurg.2020.06.018⟩ Journal of Pediatric Surgery, 2021, 56 (2), pp.337-345. ⟨10.1016/j.jpedsurg.2020.06.018⟩ |
| ISSN: | 0022-3468 1531-5037 |
| DOI: | 10.1016/j.jpedsurg.2020.06.018⟩ |
| Popis: | International audience; Background - Hirschsprung-associated enterocolitis physiopathology likely involves disturbed interactions between gut microbes and the host during the early neonatal period. Our objective was to create a neonatal porcine model of iatrogenic aganglionosis to evaluate the impact of the enteric nervous system (ENS) on microbiota and intestinal barrier postnatal development. Methods - Under general anesthesia, the rectosigmoid serosa of 5-day-old suckling piglets was exposed to 0.5% benzalkonium chloride solution (BAC, n = 7) or saline (SHAM, n = 5) for 1 h. After surgery, animals returned to their home-cage with the sow and littermates and were studied 21 days later. Results - BAC treatment induced partial aganglionosis with absence of myenteric plexus and reduced surface area of submucosal plexus ganglia (-58%, P |
| Databáze: | OpenAIRE |
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