Chiral Surface of Nanoparticles Determines the Orientation of Adsorbed Transferrin and Its Interaction with Receptors
| Autor: | Chunying Chen, Rong Lei, Yuliang Zhao, Shui Fang Zhu, Mingzhe Wang, Xinyi Wang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Stereochemistry
Surface Properties General Physics and Astronomy Nanoparticle Metal Nanoparticles Protein Corona Transferrin receptor 02 engineering and technology 010402 general chemistry Ligands 01 natural sciences Adsorption Receptors Transferrin Humans General Materials Science chemistry.chemical_classification Liposome Chemistry General Engineering Transferrin 021001 nanoscience & nanotechnology 0104 chemical sciences HEK293 Cells Colloidal gold Biophysics Nanoparticles Gold 0210 nano-technology Chirality (chemistry) |
| Zdroj: | ACS nano. 11(5) |
| ISSN: | 1936-086X |
| Popis: | When nanoparticles are exposed to a physiological environment, a "protein corona" is formed that greatly determines their biological fate. Adsorption of proteins could be influenced by chiral surfaces of nanoparticles; however, very few quantitative studies are available on the interaction of protein with the chiral surface of nanoparticles, and the underlying mechanism remains largely unresolved. We have developed a strategy to quantitatively analyze the adsorption and conformational features of transferrin on gold nanoparticles that are functionalized with d, l, and racemic penicillamine. We used a quartz microbalance platform to monitor the interaction of the adsorbed transferrin with transferrin receptors in HEK cell-derived liposomes. Results show that the chiral surface of nanoparticle determines the orientation and conformation of transferrin, which subsequently affects the interaction and recognition of transferrin with its receptor on the cellular membrane. Transferrin is widely used as a tumor-targeting ligand in cancer treatment and diagnosis since the transferrin receptor is overexpressed on the cell membrane of various types of cancer cells. Thus, the present results will help to expand the knowledge on biological identity of nanoparticles with chiral surfaces in a physiological environment and provide an insight into the rational design of therapeutic nanoparticles. |
| Databáze: | OpenAIRE |
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