The cAMP-dependent protein kinase A pathway perturbs autophagy and plays important roles in development and virulence of Sclerotinia sclerotiorum
Autor: | Pei-Ling Yu, Jeffrey A. Rollins |
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Rok vydání: | 2022 |
Předmět: |
0106 biological sciences
0303 health sciences Appressorium Virulence Autophagy Sclerotinia sclerotiorum Adenylate kinase Biology biology.organism_classification Cyclic AMP-Dependent Protein Kinases 01 natural sciences Cyclase Cell biology 03 medical and health sciences Infectious Diseases Ascomycota Genetics Signal transduction Protein kinase A Ecology Evolution Behavior and Systematics 030304 developmental biology 010606 plant biology & botany |
Zdroj: | Fungal Biology. 126:20-34 |
ISSN: | 1878-6146 |
Popis: | Previous research has demonstrated that sclerotia production is suppressed by exogenous cyclic AMP (cAMP) in Sclerotinia sclerotiorum and enhanced upon deletion of the adenylate cyclase gene. This study focuses on further functionally characterizing the cAMP-dependent protein kinase A (PKA) signaling pathway in S. sclerotiorum. Here, we demonstrate functions for two components of cAMP signaling: the catalytic, SsPKA, and the regulatory, SsPKAR, subunits of cAMP-dependent PKA. Growth and virulence were greatly reduced by disruption of either Sspka2 or SspkaR in addition to deficiencies in appressorium development. Surprisingly, disruption of both Sspka2 (dSspka2) and SspkaR (dSspkaR) display an up-regulation of autophagy without nutrient starvation suggesting that properly regulated PKA activity is required for control of autophagy. SsPKAR is demonstrated to be required for carbohydrate metabolism and mobilization, which are required for appressorium development and sclerotium initiation. A closer examination of dSspkaR during Nicotiana benthamiana infection revealed that an oxalic acid (OA)-independent necrosis protein(s) or metabolite(s) may be involved in the lesion development in dSspkaR-N. benthamiana interaction. In summary, these data demonstrate that the cAMP-dependent PKA signaling is essential for multiple forms of S. sclerotiorum development as well as virulence which rely on optimal regulation of autophagy. |
Databáze: | OpenAIRE |
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