The use of corneal organ culture in biocompatibility studies
Autor: | Sarah F. R. Taylor, Margaret D. M. Evans, Ruo Zhong Xie, Gail A. McFarland, John S. Wilkie, Hassan Chaouk |
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Rok vydání: | 2002 |
Předmět: |
Pathology
medicine.medical_specialty Materials science Time Factors Biocompatibility Polymers Biophysics Bioengineering Biocompatible Materials Organ culture Biomaterials Cornea Organ Culture Techniques Cell Movement Ophthalmology medicine Animals Corneal epithelium Fluorocarbons Wound Healing Polycarboxylate Cement Perfluoropolyether Histology Epithelial Cells Epithelium Kinetics medicine.anatomical_structure Mechanics of Materials Ceramics and Composites Microscopy Electron Scanning Cattle Wound healing Ethers |
Zdroj: | Biomaterials. 23(5) |
ISSN: | 0142-9612 |
Popis: | This study investigated the potential of a corneal organ culture system in the evaluation of polymers for ophthalmic devices that require epithelialisation. Two different polymers were tested in lenticule form to explore the sensitivity of this in vitro assay. Polycarbonate and perfluoropolyether-based lenticules were surgically implanted into bovine corneas and compared with a parallel series of sham-wounded corneas. Following surgery, all corneas were maintained in an air/liquid organ culture system for up to 8 days during which time they were evaluated clinically to monitor the rate of epithelial growth across the lenticule surface (implanted) or wound bed (sham). Data showed differences in the kinetics of epithelial migration according to the underlying surface with full epithelialisation of the sham series occurring on day 5±0.5, the perfluoropolyether lenticules on day 6±0.5 and polycarbonate lenticules on day 8±0.5. Histology revealed differences in the structure and morphology of the migrating and stable epithelium in each series of corneas. The differential response of the corneal epithelium was related to the physiochemical characteristics of the natural (sham) or synthetic (perfluoropolyether or polycarbonate) substrata which the epithelium could detect when maintained in organ culture. This assay system has utility for screening candidate polymers for certain ophthalmic applications. |
Databáze: | OpenAIRE |
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