Genetic assessment of Staphylococcus aureus in an underreported locality: Ambulatory care clinic
| Autor: | James F Schaus, Michael Deichen, Alexander M. Cole, John Deichen, Christopher L. Parkinson, Gowrishankar Muthukrishnan, Matthew F. Lawrance |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Staphylococcus aureus Genotype Population structure Population genetics Locus (genetics) Biology medicine.disease_cause lcsh:Infectious and parasitic diseases 03 medical and health sciences Ambulatory care Environmental health medicine Ambulatory Care Humans lcsh:RC109-216 Phylogeny Bacteriological Techniques Cross Infection Molecular Epidemiology Phylogenetic tree lcsh:Public aspects of medicine Public Health Environmental and Occupational Health Genetic Variation lcsh:RA1-1270 General Medicine Staphylococcal Infections Phylogenetic reconstruction Community-Acquired Infections 030104 developmental biology Infectious Diseases Florida Multilocus sequence typing Female Multilocus Sequence Typing |
| Zdroj: | Journal of Infection and Public Health, Vol 11, Iss 5, Pp 648-656 (2018) |
| ISSN: | 1876-035X |
| Popis: | Background: Staphylococcus aureus has strong association with anthropogenic environments. This association has not been well supported by use of genetic tools. The aim of this study was to phylogenetically relate numerous isolates from three environments — NCBI samples from hospitals, a community, and a previously unexplored healthcare environment: an ambulatory care clinic (ACC). Methods: This study incorporated hospital samples from NCBI, a community database from the University of Central Florida (UCF), and newly added samples taken from employees of an ambulatory care clinic located at UCF. Samples were collected from nasal swabs of employees, and positive samples were cultured, extracted, and sequenced at seven MLST loci and one virulence locus (spa). MLST sequences were used in eBURST and TCS population structure analyses and all sequences were incorporated into a phylogenetic reconstruction of relationships. Results: A total of 185 samples were incorporated in this study (15 NCBI sequences from hospital infections, 29 from the ACC, and 141 from the community). In both phylogenetic and population genetics analyses, samples proved to be panmixic, with samples not segregating monophyletically based on sample origin. Conclusion: Samples isolated from ambulatory care clinics are not significantly differentiated from either community or hospital samples at the representative loci chosen. These results strengthen previous conclusions that S. aureus may exhibit high genetic similarity across anthropogenic environments. Keywords: Staphylococcus aureus, Phylogenetic reconstruction, Population genetics, Medical environment |
| Databáze: | OpenAIRE |
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