From Bench to Biomolecular Simulation: Phospholipid Modulation of Potassium Channels
| Autor: | Daniel Quetschlich, Tanadet Pipatpolkai, Phillip J. Stansfeld |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Potassium Channels
Potassium Phospholipid chemistry.chemical_element Review Article protein-lipid interactions 03 medical and health sciences chemistry.chemical_compound Membrane Lipids 0302 clinical medicine Structural Biology Humans Super-resolution microscopy Molecular Biology Ion channel Phospholipids 030304 developmental biology ComputingMethodologies_COMPUTERGRAPHICS mass spectrometry X-ray crystallography 0303 health sciences phosphatidylinositol-bisphosphate Conductance molecular dynamics simulations electrophysiology Resting potential QP Potassium channel Cryo-EM microscopy Electrophysiology chemistry Structural biology flux assays Biophysics K+ channels 030217 neurology & neurosurgery |
| Zdroj: | Journal of Molecular Biology |
| ISSN: | 1089-8638 0022-2836 |
| Popis: | Graphical abstract Highlights • Interactions of lipids with K+ channels. • Structural basis of PIP2 binding. • Experimental methods for quantifying PIP2-binding to K+ channels. • Molecular simulation methods for studying PIP2 binding. Potassium (K+) ion channels are crucial in numerous cellular processes as they hyperpolarise a cell through K+ conductance, returning a cell to its resting potential. K+ channel mutations result in multiple clinical complications such as arrhythmia, neonatal diabetes and migraines. Since 1995, the regulation of K+ channels by phospholipids has been heavily studied using a range of interdisciplinary methods such as cellular electrophysiology, structural biology and computational modelling. As a result, K+ channels are model proteins for the analysis of protein-lipid interactions. In this review, we will focus on the roles of lipids in the regulation of K+ channels, and how atomic-level structures, along with experimental techniques and molecular simulations, have helped guide our understanding of the importance of phospholipid interactions. |
| Databáze: | OpenAIRE |
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