| Autor: |
Diethard K. Bohme, Janna Anichina, Bradley B Schneider, Thomas R. Covey, Antony D. St-Jacques |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Analytical chemistry. 82(14) |
| ISSN: |
1520-6882 |
| Popis: |
Differential mobility spectrometry has been applied to reveal the occurrence of isomerization of thymine nucleobase and of thymine dideoxynucleotide d(5'-TT-3') due to bond redisposition induced by UV irradiation at 254 nm of frozen aqueous solutions of these molecules. Collision-induced dissociation (CID) spectra of electrosprayed photoproducts of the thymine solution suggest the presence of two isomers (the so-called cyclobutane and 6,4-photoproducts) in addition to the proton-bound thymine dimer, and these were separated using differential mobility spectrometry/mass spectrometry (DMS/MS) techniques with water as the modifier. Similar experiments with d(5'-TT-3') revealed the formation of a new isomer of deprotonated thymine dideoxynucleotide upon UV irradiation that was easily distinguished using DMS/MS with isopropanol as the modifier. The results reinforce the usefulness of DMS/MS in isomer separation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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