An alternative mechanism of early nodal clustering and myelination onset in GABAergic neurons of the central nervous system

Autor: François-Xavier Lejeune, Anne-Laure Dubessy, Quentin Rappeneau, Sean A. Freeman, Julien Tailleur, Catherine Lubetzki, Nathalie Sol-Foulon, Anne Desmazières, Marie-Stéphane Aigrot, Melina Thetiot, Thomas Roux
Přispěvatelé: Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut de la Vision, Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Matière et Systèmes Complexes (MSC (UMR_7057)), Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), sol-foulon, nathalie, Institut du Cerveau = Paris Brain Institute (ICM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Central Nervous System
β2Nav
nodes of Ranvier
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Central nervous system
axonal trafficking
Hippocampal formation
Biology
dynactin-1
Cellular and Molecular Neuroscience
03 medical and health sciences
Myelin
0302 clinical medicine
Live cell imaging
Ranvier's Nodes
voltage-gated sodium channel
Molecular motor
medicine
Animals
Cluster Analysis
GABAergic Neurons
Myelin Sheath
030304 developmental biology
0303 health sciences
Node of Ranvier
Saltatory conduction
[SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
myelination
kinesin-1
live imaging
Oligodendrocyte
Axons
molecular motor
030104 developmental biology
medicine.anatomical_structure
Neurology
nervous system
GABAergic
NODAL
Neuroscience
cell adhesion molecule
030217 neurology & neurosurgery
Zdroj: Glia
Glia, Wiley, 2020, 68 (9), pp.1891-1909. ⟨10.1002/glia.23812⟩
Glia, 2020, 68 (9), pp.1891-1909. ⟨10.1002/glia.23812⟩
ISSN: 0894-1491
1098-1136
DOI: 10.1101/763573
Popis: SUMMARYIn vertebrates, fast saltatory conduction along myelinated axons relies on the node of Ranvier. How nodes assemble on CNS neurons is not yet fully understood. We recently highlighted that clusters similar to nodes can form prior to myelin deposition in hippocampal GABAergic neurons and are associated with increased conduction velocity. Here, we used a live imaging approach to characterize the intrinsic mechanisms underlying the assembly of these early clusters. We first demonstrated that their components can partially pre-assemble prior to membrane targeting and determined the molecular motors involved in their trafficking. We then demonstrated the key role of the protein β2Nav for clustering initiation. We further unraveled the fate of these early clusters, by showing that they participate in node formation, but also have an unexpected role in guiding oligodendrocyte processes prior to myelin deposition. Altogether our results shed light on an alternative mechanism of nodal clustering and myelination onset.
Databáze: OpenAIRE
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