Secreted frizzled-related protein 4 inhibits proliferation and metastatic potential in prostate cancer
| Autor: | C. Soon Lee, Darren N. Saunders, Jayne E. Lelliott, John J. Grygiel, Susan M. Henshall, Robert L. Sutherland, Lisa G. Horvath, Elizabeth D. Williams, James G. Kench |
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| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty Frizzled Urology Biology urologic and male genital diseases Metastasis Prostate cancer Prostate Internal medicine Cell Line Tumor Proto-Oncogene Proteins LNCaP medicine Humans Neoplasm Invasiveness Neoplasm Metastasis beta Catenin Cell Proliferation Wnt signaling pathway Prostatic Neoplasms medicine.disease Cadherins Androgen receptor Gene Expression Regulation Neoplastic Wnt Proteins medicine.anatomical_structure Endocrinology Phenotype Oncology Receptors Androgen Cancer research SFRP4 Signal Transduction |
| Zdroj: | The Prostate. 67(10) |
| ISSN: | 0270-4137 |
| Popis: | BACKGROUND Secreted frizzled-related proteins (sFRP4) inhibits Wnt signaling and thus cellular proliferation in androgen-independent prostate cancer cells in vitro. However, increased expression of membranous sFRP4 is associated with a good prognosis in human localized androgen-dependent prostate cancer, suggesting a role for sFRP4 in early stage disease. Here, we investigated the phenotype of sFRP4 overexpression in an androgen-dependent prostate cancer model. METHODS An sFRP4-overexpressing androgen-dependent (LNCaP) prostate cancer model was established to assess changes in cellular proliferation, the expression, and subcellular localization of adhesion molecules and cellular invasiveness, and compared with the findings in sFRP4-overexpressing androgen-independent cells (PC3). RESULTS sFRP4 overexpression in both cell line models resulted in a morphologic change to a more epithelioid cell type with increased localization of β-catenin and cadherins (E-cadherin in LNCaP, N-cadherin in PC3) to the cell membrane. Functionally, sFRP4 overexpression was associated with a decreased rate of proliferation (P = 0.0005), decreased anchorage-independent growth (P |
| Databáze: | OpenAIRE |
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