EGFR mutations in non-small cell lung cancer: an audit from West China Hospital
| Autor: | Wei-Ya Wang, Wenyan Zhang, Ke Zheng, Jie Chen, Xueying Su, Lili Jiang, Yan Zou, Weiping Liu, Yuan Tang |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine China Pathology medicine.medical_specialty Lung Neoplasms DNA Mutational Analysis Cell Mutant non-small cell lung cancer (NSCLC) Biology medicine.disease_cause Pathology and Forensic Medicine 03 medical and health sciences Exon 0302 clinical medicine Carcinoma Non-Small-Cell Lung Genetics medicine Humans Lung cancer Molecular Biology Neoplasm Staging Medical Audit Mutation Incidence (epidemiology) Histology Exons Sequence Analysis DNA medicine.disease ErbB Receptors 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Cancer research Molecular Medicine Female Neoplasm Grading |
| Zdroj: | Expert Review of Molecular Diagnostics. 16:915-919 |
| ISSN: | 1744-8352 1473-7159 |
| DOI: | 10.1080/14737159.2016.1199961 |
| Popis: | Objectives: To discover the incidence and characteristics of EGFR mutations in non-small cell lung cancer (NSCLC) in a single, large cohort as a part of routine diagnostic investigations. Methods: We reviewed EGFR mutations investigated by Amplification Refractory Mutation System (ARMS) PCR (covering 29 known mutations) using DNA samples from FFPE tissue or cell clot specimens in a total of 3894 cases of NSCLC analysed between 2012-2014. Results: EGFR mutations are preferentially associated with adenocarcinomaand adenosquamous histology, particularly those well to moderately differentiated, and were significantly more common in female than male patients irrespective of histological subtypes. Exon 19 deletion (45.7%) and exon 21 L858R (45.6%) accounted for the vast majority of the EGFR mutations detected, with the remaining mutations being infrequent (Conclusion: Our data represents the largest EGFR mutation survey based on routine clinical diagnostic laboratory data from a single institution, it confirms the incidence and characteristics of EGFR mutations in NSCLC seen in Asian patients, and also unravels the combinatorial nature of rare compound EGFR mutations. |
| Databáze: | OpenAIRE |
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