Single-Step LRET Aptasensor for Rapid Mycotoxin Detection
Autor: | Doyeon Bang, Ju-Young Byun, Eun-Jung Jo, Jae Young Lee, Jun Ho Son, Luke P. Lee, Hyoyoung Mun, Min-Gon Kim |
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Rok vydání: | 2017 |
Předmět: |
Luminescent Agents
Quenching (fluorescence) Luminescence resonance energy transfer Infrared Rays Aptamer 010401 analytical chemistry Metal Nanoparticles Food Contamination Nanotechnology Single step 02 engineering and technology Aptamers Nucleotide Mycotoxins 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Upconversion nanoparticles chemistry Limit of Detection Luminescent Measurements 0210 nano-technology Mycotoxin |
Zdroj: | Analytical Chemistry. 90:716-722 |
ISSN: | 1520-6882 0003-2700 |
Popis: | Contamination of foods by mycotoxins is a common yet serious problem. Owing to the increase in consumption of fresh produce, consumers have become aware of food safety issues caused by mycotoxins. Therefore, rapid and sensitive mycotoxin detection is in great demand in fields such as food safety and public health. Here we report a single-step luminescence resonance energy transfer (LRET) aptasensor for mycotoxin detection. To accomplish the single-step sensor, our sensor was constructed by linking a quencher-labeled aptamer through a linker to the surface of upconversion nanoparticles (UCNPs). Our LRET aptasensor is composed of Mn2+-doped NaYF4:Yb3+,Er3+ UCNPs as the LRET donor, and black hole quencher 3 (BHQ3) as the acceptor. The maximum quenching efficiency is obtained by modulating the linker length, which controls the distance between the quencher and the UCNPs. Our distinctive design of LRET aptasensor allows detection of mycotoxins selectively in colored food samples within 10 min without multiple ... |
Databáze: | OpenAIRE |
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