Evaluation of Commonly Used Serological Tests for Detection of Coxiella burnetii Antibodies in Well-Defined Acute and Follow-Up Sera
| Autor: | Cornelia C. H. Wielders, Marion Koopmans, M. C. A. Wegdam-Blans, H.A. Bijlmer, Tineke Herremans, J. M. Korbeeck, Peter M. Schneeberger, Jamie C. E. Meekelenkamp, H. T. Tjhie |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Microbiology (medical) Time Factors Clinical Biochemistry Immunology Enzyme-Linked Immunosorbent Assay Q fever Immunoglobulin G Serology Diagnostic Laboratory Immunology parasitic diseases medicine Humans Immunology and Allergy Fluorescent Antibody Technique Indirect Direct fluorescent antibody Aged biology Middle Aged bacterial infections and mycoses medicine.disease Complement fixation test Coxiella burnetii biology.organism_classification Antibodies Bacterial Virology Immunoglobulin M biology.protein Female Antibody Q Fever |
| Zdroj: | Clinical and Vaccine Immunology. 19:1110-1115 |
| ISSN: | 1556-679X 1556-6811 |
| Popis: | In this study, we comparedCoxiella burnetiiIgG phase I, IgG phase II, and IgM phase II detection among a commercially available enzyme-linked immunosorbent assay (ELISA) (Virion/Serion), an indirect fluorescent antibody test (IFAT) (Focus Diagnostics), and a complement fixation test (CFT) (Virion/Serion). For this, we used a unique collection of acute- and convalescent-phase sera from 126 patients with acute Q fever diagnosed by positiveCoxiella burnetiiPCR of blood. We were able to establish a reliable date of onset of disease, since DNA is detectable within 2 weeks after the start of symptoms. In acute samples, att= 0, IFAT demonstrated IgM phase II antibodies in significantly more sera than did ELISA (31.8% versus 19.7%), although the portion of solitary IgM phase II was equal for IFAT and for ELISA (18.2% and 16.7%, respectively). Twelve months after the diagnosis of acute Q fever, 83.5% and 62.2% of the sera were still positive for IgM phase II with IFAT and ELISA, respectively. At 12 months IFAT IgG phase II showed the slowest decline. Therefore, definitive serological evidence of acute Q fever cannot be based on a single serum sample in areas of epidemicity and should involve measurement of both IgM and IgG antibodies in paired serum. Based on IgG phase II antibody detection in paired samples (at 0 and 3 months) from 62 patients, IFAT confirmed more cases than ELISA and CFT, but the differences were not statically significant (100% for IFAT, 95.2% for ELISA, and 96.8% for CFT). This study demonstrated that the three serological tests are equally effective in diagnosing acute Q fever within 3 months of start of symptoms. In follow-up sera, more IgG antibodies were detected by IFAT than by ELISA or CFT, making IFAT more suitable for prevaccination screening programs. |
| Databáze: | OpenAIRE |
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