Kinetics of Eotaxin Generation and Its Relationship to Eosinophil Accumulation in Allergic Airways Disease: Analysis in a Guinea Pig Model In Vivo
| Autor: | Roger T. Palframan, Amanda E. I. Proudfoot, Sara M. Rankin, Sylvie Marleau, David A. Griffiths-Johnson, Peter J. Jose, Timothy N. C. Wells, Dolores M. Conroy, Peter K. Jeffery, Alison A. Humbles, Dechun Li, Timothy J. Williams |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Chemokine CCL11
Male Eotaxin Pathology medicine.medical_specialty Chemotactic Factors Eosinophil Guinea Pigs Immunology Bone Marrow Cells Dexamethasone Article 03 medical and health sciences 0302 clinical medicine immune system diseases medicine Animals Immunology and Allergy Eosinophilia Chemoattractant activity Lung Interleukin 5 Serum Albumin 030304 developmental biology 0303 health sciences medicine.diagnostic_test biology business.industry respiratory system Eosinophil Asthma respiratory tract diseases 3. Good health Eosinophils Ovalbumin Bronchoalveolar lavage medicine.anatomical_structure Chemokines CC biology.protein Cytokines Respiratory epithelium Female Interleukin-5 medicine.symptom business Bronchoalveolar Lavage Fluid 030215 immunology |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| DOI: | 10.1084/jem.186.4.601 |
| Popis: | Challenge of the airways of sensitized guinea pigs with aerosolized ovalbumin resulted in an early phase of microvascular protein leakage and a delayed phase of eosinophil accumulation in the airway lumen, as measured using bronchoalveolar lavage (BAL). Immunoreactive eotaxin levels rose in airway tissue and BAL fluid to a peak at 6 h falling to low levels by 12 h. Eosinophil numbers in the tissue correlated with eotaxin levels until 6 h but eosinophils persisted until the last measurement time point at 24 h. In contrast, few eosinophils appeared in BAL over the first 12 h, major trafficking through the airway epithelium occurring at 12–24 h when eotaxin levels were low. Constitutive eotaxin was present in BAL fluid. Both constitutive and allergen-induced eosinophil chemoattractant activity in BAL fluid was neutralized by an antibody to eotaxin. Allergen-induced eotaxin appeared to be mainly in airway epithelium and macrophages, as detected by immunostaining. Allergen challenge of the lung resulted in a rapid release of bone marrow eosinophils into the blood. An antibody to IL-5 suppressed bone marrow eosinophil release and lung eosinophilia, without affecting lung eotaxin levels. Thus, IL-5 and eotaxin appear to cooperate in mediating a rapid transfer of eosinophils from the bone marrow to the lung in response to allergen challenge. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|