Activation of latent transgenes in Arabidopsis using a hybrid transcription factor
| Autor: | Sabrina Rouse, Sandra L. Volrath, Ann Tuttle, Steve Goff, Lyle Crossland, Dave Guyer, Johnson Marie A, Jörn Görlach, Potter Sharon L, Eric R. Ward |
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| Rok vydání: | 1998 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Recombinant Fusion Proteins Arabidopsis Saccharomyces cerevisiae Biology Fungal Proteins Transformation Genetic Transcription (biology) Gene expression Genetics Transgenes Transcription factor Gene Crosses Genetic Reporter gene Effector fungi Promoter biology.organism_classification Plants Genetically Modified Molecular biology DNA-Binding Proteins Gene Expression Regulation Rhizobium Transcription Factors Research Article |
| Zdroj: | Genetics. 149(2) |
| ISSN: | 0016-6731 |
| Popis: | A hybrid transcription factor comprising a fusion of the DNA-binding domain of Saccharomyces cerevisiae GAL4 and the transcription activation domain of maize C1 was expressed in stably transformed Arabidopsis. Additional transgenic lines were created containing test genes controlled by a synthetic promoter consisting of concatemeric copies of the cis-acting site recognized by GAL4 (UASG) fused to a minimal promoter. The GAL4/C1 effector line was crossed to two lines containing a synthetic promoter/GUS fusion. Both histochemical staining and GUS activity assays indicate strong activation of GUS expression was achieved only after crossing. The GAL4/C1 effector line was also crossed to 15 lines containing a synthetic promoter/antisense adenylosuccinate synthetase gene. Severely retarded growth, and in some cases lethality, was observed in 40% of the F1 lines. This system of activation by crossing is generally useful for activating expression of test transgenes. |
| Databáze: | OpenAIRE |
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