Induced thyme product prevents VEGF-induced migration in human umbilical vein endothelial cells
| Autor: | John Madden, Kevin Huncik, Diane Krill, Peter D. R. Moeller |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Vascular Endothelial Growth Factor A
Umbilical Veins Angiogenesis Biophysics Neovascularization Physiologic Angiogenesis Inhibitors Pharmacology Biology Biochemistry Umbilical vein Thymus Plant Neovascularization Dogs Cell Movement In vivo medicine Animals Humans Molecular Biology Cells Cultured Chromatography High Pressure Liquid Vascular tissue Methanol Endothelial Cells Cell Biology Vascular endothelial growth factor A medicine.anatomical_structure Cell culture Immunology Drug Screening Assays Antitumor medicine.symptom Blood vessel |
| Zdroj: | Biochemical and Biophysical Research Communications. 403:275-281 |
| ISSN: | 0006-291X |
| Popis: | Compounds with anti-angiogenic properties are useful in combating cancer by preventing new blood vessel formation to support the tumor. In this report we introduce a rapid method for screening potential anti-angiogenic compounds in a model system that stimulates the production of secondary defense chemicals in plants. This methodology identified an inducible vascular factor (IVF3), which was found to be inhibitory in all of the model systems tested. Thyme plants were exposed to highly vascular mint plants and the methanol extracts were analyzed by reverse phase HPLC. The thyme compounds induced by the invading mint tissue, and not present in the thyme plants grown alone, were tested in a vertical plate assay measuring root length as a quantitative assay for drug sensitivity. The HPLC-purified extract, referred to as IVF3, reduced the growth of root vascular tissue compared to the control and vehicle control, and 50% as well as known angiogenesis inhibitors, VEGF receptor tyrosine kinase inhibitor and amiloride hydrochloride. Extracted compounds that were effective inhibitors of plant roots were assayed in Madin Darby canine kidney epithelial cells (MDCK) for toxicity, and in human umbilical vein endothelial cells (HUVEC) for their effect on migration. IVF3 was effective at limiting HUVEC migration in VEGF-stimulated cultures. In vivo video capture of intersegmental vessel circulation between 48 and 72 h post fertilization in the developing vasculature of zebrafish embryos showed IVF3 also significantly reduced ISV functional circulation. This report demonstrates the anti-angiogenic effects of IVF3 extract in endothelial cells and in an intact vertebrate model for angiogenesis. |
| Databáze: | OpenAIRE |
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