Transcriptomic investigation reveals donor-specific gene signatures in human lung transplants
| Autor: | Shaf Keshavjee, A.T. Sage, Ricardo Zamel, Xiao-Hui Bai, Mamatha Bhat, Marcelo Cypel, Cristina Baciu, Olivia Hough, Mingyao Liu, Jason Shin, Jonathan C. Yeung |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine Extracorporeal Circulation Programmed cell death Necrosis Microarray Proinflammatory cytokine Transcriptome 03 medical and health sciences 0302 clinical medicine medicine Humans Lung business.industry Tissue Donors 3. Good health Perfusion Transplantation Gene expression profiling 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Cancer research medicine.symptom business Lung Transplantation |
| Zdroj: | European Respiratory Journal. 57:2000327 |
| ISSN: | 1399-3003 0903-1936 |
| DOI: | 10.1183/13993003.00327-2020 |
| Popis: | IntroductionTransplantation of lungs from donation after circulatory death (DCD) in addition to donation after brain death (DBD) became routine worldwide to address the global organ shortage. The development ofex vivolung perfusion (EVLP) for donor lung assessment and repair contributed to the increased use of DCD lungs. We hypothesise that a better understanding of the differences between lungs from DBD and DCD donors, and between EVLP and directly transplanted (non-EVLP) lungs, will lead to the discovery of the injury-specific targets for donor lung repair and reconditioning.MethodsTissue biopsies from human DBD (n=177) and DCD (n=65) donor lungs, assessed with or without EVLP, were collected at the end of cold ischaemic time. All samples were processed with microarray assays. Gene expression, network and pathway analyses were performed using R, Ingenuity Pathway Analysis and STRING. Results were validated with protein assays, multiple logistic regression and 10-fold cross-validation.ResultsOur analyses showed that lungs from DBD donors have upregulation of inflammatory cytokines and pathways. In contrast, DCD lungs display a transcriptome signature of pathways associated with cell death, apoptosis and necrosis. Network centrality revealed specific drug targets to rehabilitate DBD lungs. Moreover, in DBD lungs, tumour necrosis factor receptor-1/2 signalling pathways and macrophage migration inhibitory factor-associated pathways were activated in the EVLP group.A panel of genes that differentiate the EVLP from the non-EVLP group in DBD lungs was identified.ConclusionThe examination of gene expression profiling indicates that DBD and DCD lungs have distinguishable biological transcriptome signatures. |
| Databáze: | OpenAIRE |
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