Experimental infection of Artibeus intermedius bats with serotype-2 dengue virus
| Autor: | L Perea-Martinez, M M Moreno-Altamirano, Mónica Salas-Rojas, Noël Tordo, P H Marianneau, Nidia Aréchiga-Ceballos, Hayde Moreno-Sandoval, M. M. García-Flores, Alvaro Aguilar-Setién |
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| Rok vydání: | 2012 |
| Předmět: |
Serotype
Male animal structures viruses Immunology Artibeus intermedius Dengue virus Biology medicine.disease_cause Antibodies Viral Microbiology Virus Dengue fever Dengue Chiroptera medicine Immunology and Allergy Animals Aedes Hematoma General Veterinary Reverse Transcriptase Polymerase Chain Reaction General Medicine Dengue Virus Viral Load biology.organism_classification medicine.disease Virology Flavivirus Infectious Diseases RNA Viral Female Viral load Spleen |
| Zdroj: | Comparative immunology, microbiology and infectious diseases. 36(2) |
| ISSN: | 1878-1667 |
| Popis: | Dengue fever is caused by a flavivirus that primarily infects humans and Aedes sp. mosquitoes. However, viral replication in wild animals other than non-human primates has been scarcely studied. In this report, the susceptibility of Artibeus intermedius frugivorous bat to serotype-2 dengue virus (DENV-2) infection was tested. Twenty-three bats were intraperitoneally inoculated with different viral loads of DENV-2 (New Guinea-C strain). Forty-three percent of the infected bats developed bruises on the chest or on the wings. Histological analyses showed structural alterations in the spleen and bleeding in liver and intestine, but the virus was not detected by RT-PCR in any of the analyzed tissues, and it was found in only one bat (kidney) by semi-nested RT-PCR. In sera, the viral RNA was detected by semi-nested RT-PCR in 39% of bats, but only 8% of bats seroconverted. Overall, these data indicate that DENV-2 replicates poorly in these bats, suggesting they are not suitable hosts to this virus. |
| Databáze: | OpenAIRE |
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