Effects of intramammary infection on whey proteinograms of sheep during lactation
| Autor: | Eduardo Levi de Sousa Guaraná, José Jurandir Fagliari, Carla Lopes de Mendonça, Paulo César da Silva, Vânia F. Lemos, Pierre Castro Soares, José Augusto Bastos Afonso |
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| Přispěvatelé: | Universidade Federal Rural de Pernambuco (UFRPE), UFRPE, Clínica de Bovinos, UFRPE, Campus Garanhuns, Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2015 |
| Předmět: |
Subclinical mastitis
medicine.medical_specialty Whey protein Globulin Mammary gland Biology Microbiology acute-phase protein Internal medicine Lactation leite medicine Mastite subclínica Beta-lactoglobulin ovelhas milk lcsh:Veterinary medicine General Veterinary Lactoferrin Haptoglobin Acute-phase protein marcadores biológicos ewes food and beverages biomarkers Milk Endocrinology medicine.anatomical_structure biology.protein lcsh:SF600-1100 proteína de fase aguda Ewes Biomarkers |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Pesquisa Veterinária Brasileira, Vol 35, Iss 3, Pp 230-236 Pesquisa Veterinária Brasileira, Vol 35, Iss 3, Pp 230-236 (2015) Pesquisa Veterinária Brasileira v.35 n.3 2015 Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA Pesquisa Veterinária Brasileira, Volume: 35, Issue: 3, Pages: 230-236, Published: MAR 2015 |
| ISSN: | 0100-736X |
| DOI: | 10.1590/s0100-736x2015000300004 |
| Popis: | The study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), α-lactalbumin, β-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and α-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P |
| Databáze: | OpenAIRE |
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