Pleurotus citrinopileatus polysaccharide stimulates anti-inflammatory properties during monocyte-to-macrophage differentiation
| Autor: | Lisa C. Laan, Masashi Mizuno, Irma van Die, Ken-ichiro Minato |
|---|---|
| Přispěvatelé: | VU University medical center, Molecular cell biology and Immunology, AGEM - Digestive immunity, AII - Inflammatory diseases |
| Rok vydání: | 2019 |
| Předmět: |
Lipopolysaccharides
Chemokine medicine.medical_treatment Anti-Inflammatory Agents 02 engineering and technology CCL2 Pleurotus Biochemistry Monocytes Cell Line Microbiology Interferon-gamma 03 medical and health sciences Immune system Structural Biology medicine Humans Lectins C-Type Molecular Biology 030304 developmental biology 0303 health sciences Innate immune system biology Chemistry Macrophages Monocyte Cell Differentiation Fungal Polysaccharides General Medicine 021001 nanoscience & nanotechnology TLR2 Phenotype medicine.anatomical_structure Cytokine biology.protein Tumor necrosis factor alpha Chemokines 0210 nano-technology Signal Transduction |
| Zdroj: | Minato, K, Laan, L C, van Die, I & Mizuno, M 2019, ' Pleurotus citrinopileatus polysaccharide stimulates anti-inflammatory properties during monocyte-to-macrophage differentiation ', International Journal of Biological Macromolecules, vol. 122, pp. 705-712 . https://doi.org/10.1016/j.ijbiomac.2018.10.157 International Journal of Biological Macromolecules, 122, 705-712. Elsevier |
| ISSN: | 0141-8130 |
| Popis: | Polysaccharides from edible mushrooms possess important immunomodulating effects on immune cells including monocytes and macrophages. Macrophages activated by LPS/IFNγ are polarized toward inflammatory macrophages, whereas the anti-inflammatory properties of alternative activated macrophages play an important regulatory role in the innate immune system. We here show that the Pleurotus citrinopileatus mushroom polysaccharide (PCPS) can modulate the monocyte-to-macrophage differentiation early at the monocyte stage. Using both human THP-1 monocytic cells as well as human peripheral monocytes, we showed that PCPS inhibits the secreted levels of the pro-inflammatory cytokines TNF and IL-6, after stimulation of macrophages derived from PCPS-treated monocytes, with IFNγ + LPS. In addition, the glucan induced a tendency to increase the secreted levels of the anti-inflammatory cytokine IL-10, enhanced the expression levels of CCL2 and CCL8 mRNAs, and inhibited expression of CCR2 mRNA in the IFNγ/LPS activated macrophages. Interestingly, these data suggest that PCPS can induce a long-lasting anti-inflammatory effect in monocytes. Treatment of monocytes with laminarin and antibodies against Dectin-1 and TLR2 during PCPS treatment affected the glucan-modulated macrophage differentiation. In summary, the results of this study indicate that the glucan directs the differentiation of monocytes toward a macrophage cell population with reduced pro-inflammatory capacity via Dectin-1 and TLR2. |
| Databáze: | OpenAIRE |
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