Histopathological Findings of Testicular Tissue Following Cadmium Toxicity in Rats
| Autor: | Maryam Fekri Soofi Abadi, Bahram Dabiri, Saeedeh Shojaeepour, Fatemeh Hashemi, Shahriar Dabiri, Masoud Imani |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
endocrine system
urogenital system medicine.medical_treatment Intraperitoneal injection Cadmium toxicity H&E stain Biology medicine.disease Sertoli cell Andrology medicine.anatomical_structure Giant cell Testis medicine Pathology Immunohistochemistry RB1-214 Rat Original Article Reproductive toxicity Germ cell Calcification |
| Zdroj: | Iranian Journal of Pathology Iranian Journal of Pathology, Vol 16, Iss 4, Pp 348-353 (2021) |
| ISSN: | 2345-3656 1735-5303 |
| Popis: | Background & Objective: Reproductive toxicity of cadmium (Cd) as an environmental toxicant has been proved in animals and humans. Exposure to Cd impairs testes organs and can reduce male fertility. The present study was designed to investigate the spectrum of histopathological changes in testicular tissue focusing on Sertoli cells in rats following Cd intoxication. Methods: In the present experiment, acute testicular toxicity was induced by an intraperitoneal injection of 1.2 mg/kg CdCl2 to the animals in the test group, while the control group received normal saline. After 52 days, the animals were euthanized, and testicular tissue was stained by Hematoxylin and Eosin. In addition, immunohistochemical staining was performed on Sertoli cells for Wilms' Tumor, Melan-A, and CD99 to evaluate histopathological changes. Results: Cd caused significant alterations in seminiferous tubules with varying effects on the patterns of spermatozoa production. These histopathological changes were significantly higher in the Cd group, compared to the control group. Conclusion: The Cd-induced stepwise spectrum changes included sloughing, disorganization, hypospermatogenesis, spermatic cell arrest, germ cell hypoplasia, Sertoli cell-only pattern, fibro-hyalinized seminiferous tubules, and calcification. Sertoli cells accumulated and created multinucleated giant cells in the seminiferous tubules during the atrophic process, which could be dependent upon Sertoli cells viability and function. |
| Databáze: | OpenAIRE |
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