Fabrication of a planar-form screen-printed solid electrolyte modified Ag/AgCl reference electrode for application in a potentiometric biosensor
| Autor: | Tse-Chuan Chou, Wei-Yin Liao |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Working electrode
Silver Standard hydrogen electrode Chemistry Analytical chemistry Silver Compounds Biosensing Techniques Equipment Design Hydrogen-Ion Concentration Reference electrode pH meter Glass electrode Analytical Chemistry law.invention Electrolytes Quinhydrone electrode law Saturated calomel electrode Palladium-hydrogen electrode Potentiometry Humans Electrodes Blood Chemical Analysis Food Analysis |
| Zdroj: | Analytical chemistry. 78(12) |
| ISSN: | 0003-2700 |
| Popis: | This study features the fabrication of a planar-form, solid electrolyte modified, (PSEM) Ag/AgCl reference electrode using a screen-printing method. The PSEM Ag/AgCl reference electrode uses agar gel as the inner electrolyte and chloroprene rubber for the liquid junction and insulator. These common low-cost materials and the simple fabrication processes involved render the proposed reference electrode an ideal candidate for cost-efficient mass production. It is shown that the developed reference electrode is insensitive to most of the physiologically important ionic species, including Na+, K+, Li+, Ca2+, NH4+, and Cl-, under continuous measurement conditions. Moreover, as with conventional commercial reference electrodes, the proposed reference electrode exhibits a reversible response, which is maintained until the agar gel dries out. The PSEM Ag/AgCl reference electrode is integrated with an iridium oxide modified Pt-based pH indicator electrode to form a chip-type pH biosensor. The performance of this biosensor is consistent with that obtained from a pH meter based on a macroscopic commercial Ag/AgCl reference electrode. The experimental results confirm that the proposed biosensor is capable of providing precise pH measurements of various real samples. Accordingly, the PSEM Ag/AgCl reference electrode presented in this study provides a viable alternative to the macroscopic Ag/AgCl reference electrode used in many conventional chip-based pH sensors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|