Comparative Proteomic Profiling of Ehrlichia ruminantium Pathogenic Strain and Its High-Passaged Attenuated Strain Reveals Virulence and Attenuation-Associated Proteins
| Autor: | Elisabete Pires, Markus Müller, Miguel Ventosa, Thierry Lefrançois, Ana Varela Coelho, Nathalie Vachiery, Isabel Marcelino, Frédérique Lisacek |
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| Přispěvatelé: | Instituto de Biologia Experimental e Tecnológica, Instituto de Tecnologia Química e Biológica António Xavier (ITQB), Universidade Nova de Lisboa (NOVA), Contrôle des maladies animales exotiques et émergentes (UMR CMAEE), Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Proteome Informatics, Swiss Institute of Bioinformatics, EU projects COST action [FA-1002], EPIGENESIS RegPot European project [315988], Instituto de Biologia Experimental e Tecnológica (IBET), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Swiss Institute of Bioinformatics [Lausanne] (SIB), Université de Lausanne (UNIL)-Université de Lausanne (UNIL) |
| Rok vydání: | 2015 |
| Předmět: |
Proteomics
tique Glycosylation Proteome Biodiversité et Ecologie Rickettsiales lcsh:Medicine cowdria L73 - Maladies des animaux Protéine microbienne biodiversité Pathogénèse chemistry.chemical_compound Caprin Ehrlichia ruminantium lcsh:Science protéome Chèvre 0303 health sciences Multidisciplinary Virulence analyse statistique santé animale Effector 3. Good health protéine Research Article virulent strain Hypovirulence Virulence Factors Ehrlichia Biology Microbiology Biodiversity and Ecology 03 medical and health sciences statistical analysis Bacterial Proteins maldi tof Électrophorèse sur gel Maladie transmissible par tiques 030304 developmental biology Génie génétique animal health 030306 microbiology Proteomic Profiling Intracellular parasite lcsh:R électrophorèse sds page biology.organism_classification souche virulente chemistry lcsh:Q U30 - Méthodes de recherche [SDE.BE]Environmental Sciences/Biodiversity and Ecology protein Protein Processing Post-Translational |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2015, 10 (12), 23 p. ⟨10.1371/journal.pone.0145328⟩ PLoS ONE, Vol 10, Iss 12, p e0145328 (2015) PloS One Plos One 12 (10), 23 p.. (2015) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0145328⟩ |
| Popis: | The obligate intracellular bacterium Ehrlichia ruminantium (ER) causes heartwater, a fatal tick-borne disease in livestock. In the field, ER strains present different levels of virulence, limiting vaccine efficacy, for which the molecular basis remains unknown. Moreover, there are no genetic tools currently available for ER manipulation, thus limiting the knowledge of the genes/proteins that are essential for ER pathogenesis and biology. As such, to identify proteins and/or mechanisms involved in ER virulence, we performed the first exhaustive comparative proteomic analysis between a virulent strain (ERGvir) and its high-passaged attenuated strain (ERGatt). Despite their different behaviors in vivo and in vitro, our results from 1DE-nanoLC-MS/MS showed that ERGvir and ERGatt share 80% of their proteins; this core proteome includes chaperones, proteins involved in metabolism, protein-DNA-RNA biosynthesis and processing, and bacterial effectors. Conventional 2DE revealed that 85% of the identified proteins are proteoforms, suggesting that post-translational modifications (namely glycosylation) are important in ER biology. Strain-specific proteins were also identified: while ERGatt has an increased number and overexpression of proteins involved in cell division, metabolism, transport and protein processing, ERGvir shows an overexpression of proteins and proteoforms (DIGE experiments) involved in pathogenesis such as Lpd, AnkA, VirB9 and B10, providing molecular evidence for its increased virulence in vivo and in vitro. Overall, our work reveals that ERGvir and ERGatt proteomes are streamlined to fulfill their biological function (maximum virulence for ERGvir and replicative capacity for ERGatt), and we provide both pioneering data and novel insights into the pathogenesis of this obligate intracellular bacterium. |
| Databáze: | OpenAIRE |
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