| Autor: |
Tiago Feitosa Mota, Claudia Ida Brodskyn, Luis Gustavo Morello, Fabricio Klerynton Marchini, Marco Aurelio Krieger, Rita Cássia Pontello Rampazzo, Deborah Bittencourt Mothé Fraga |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Medical and veterinary entomologyREFERENCES. 36(2) |
| ISSN: |
1365-2915 |
| Popis: |
The study aimed to develop a multiplex qPCR to detect Leishmania infantum load in different sandfly sample settings using Leishmania kDNA and sandfly vacuolar ATPase (VATP) subunit C as internal control gene. The amplification of Lutzomyia longipalpis VATP gene was evaluated together with Leishmania infantum kDNA in a multiplex reaction. The concentration of VATP gene oligonucleotides was adjusted until no statistically significant difference was observed between all multiplex standard curves and singleplex curves, that is, only kDNA amplification. Limit of detection (LoD) was measured using a probit model and a cut-off defined by receiver operating characteristic analysis. Limit of quantification (LoQ) was assessed by a linear model using the coefficient of variation threshold of 25%. After assuring VATP gene amplification, its primer-probe concentrations were best at 100 nM/10 nM, respectively. The cut-off C |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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