Accurate quantification of lipid species affected by isobaric overlap in Fourier-transform mass spectrometry
| Autor: | Ralph Burkhardt, Gerhard Liebisch, Christer S. Ejsing, Sabrina Krautbauer, Marcus Höring, Verena M. Ertl |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
HRMS high-resolution MS peak interference Fourier-transform mass spectrometry Analytical chemistry FIA flow injection analysis 610 Medizin Isotope correction Natural abundance 030204 cardiovascular system & hematology Biochemistry Fourier transform ion cyclotron resonance Lipidomics Mass spectrometry Lipids Phospholipids Sphingolipids Triglycerides Data processing Fourier-Transform mass spectrometry Peak interference PC phosphatidylcholine 0302 clinical medicine Endocrinology FWHM full-width at half maximum Abundance (ecology) Methods AGC automated gain control triglycerides mass spectrometry ddc:610 CE cholesteryl ester TG triglyceride Chemistry FTMS Fourier-transform mass spectrometry RIA relative isotopic abundance data processing LPE lysophosphatidylethanolamine Cer ceramide QD415-436 lipids 03 medical and health sciences DG diglyceride phospholipids Detection limit Flow injection analysis LOQ limit of quantification LPC lysophosphatidylcholine SM sphingomyelin sphingolipids isotope correction DB double bond Cell Biology 030104 developmental biology Isobaric process lipidomics |
| Zdroj: | Journal of Lipid Research, Vol 62, Iss, Pp 100050-(2021) Höring, M, Ejsing, C S, Krautbauer, S, Ertl, V M, Burkhardt, R & Liebisch, G 2021, ' Accurate quantification of lipid species affected by isobaric overlap in Fourier-transform mass spectrometry ', Journal of Lipid Research, vol. 62, 100050 . https://doi.org/10.1016/j.jlr.2021.100050 Journal of Lipid Research |
| ISSN: | 0022-2275 |
| DOI: | 10.1016/j.jlr.2021.100050 |
| Popis: | Lipidomics data require consideration of ions with near-identical masses, which comprises among others the Type-II isotopic overlap. This overlap occurs in series of lipid species differing only by number of double bonds (DBs) mainly because of the natural abundance of 13C-atoms. High-resolution mass spectrometry, such as Fourier-transform mass spectrometry (FTMS), is capable of resolving Type-II overlap depending on mass resolving power. In this work, we evaluated FTMS quantification accuracy of lipid species affected by Type-II overlap. Spike experiments with lipid species pairs of various lipid classes were analyzed by flow injection analysis-FTMS. Accuracy of quantification was evaluated without and with Type-II correction (using relative isotope abundance) as well as utilizing the first isotopic peak (M+1). Isobaric peaks, which were sufficiently resolved, were most accurate without Type-II correction. In cases of partially resolved peaks, we observed peak interference causing distortions in mass and intensity, which is a well-described phenomenon in FTMS. Concentrations of respective species were more accurate when calculated from M+1. Moreover, some minor species, affected by considerable Type-II overlap, could only be quantified by M+1. Unexpectedly, even completely unresolved peaks were substantially overcorrected by Type-II correction because of peak interference. The described method was validated including intraday and interday precisions for human serum and fibroblast samples. Taken together, our results show that accurate quantification of lipid species by FTMS requires resolution-depended data analysis. |
| Databáze: | OpenAIRE |
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