Altered B Cell Homeostasis in Patients with Major Depressive Disorder and Normalization of CD5 Surface Expression on Regulatory B Cells in Treatment Responders
| Autor: | Volker Arolt, Katja Koelkebeck, Silke Jörgens, Mehrdad Rahbar Kooybaran, Julia Scheffer, Stefanie Scheu, Christian Bürger, Vladislava Falcone, Laura Grosse, Katharina Seiler, Kathrin Schwarte, Fernand Roos, Judith Alferink, Udo Dannlowski, Diana Ahmetspahic, Oliver Ambrée |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Regulatory B cells Immunology Neuroscience (miscellaneous) B-Lymphocyte Subsets Pilot Projects CD5 Antigens 03 medical and health sciences 0302 clinical medicine Immune system B cell homeostasis medicine Immunology and Allergy Homeostasis Humans B cell Pharmacology B-Lymphocytes Regulatory Depressive Disorder Major biology CD24 Germinal center Middle Aged 030104 developmental biology medicine.anatomical_structure CD1D biology.protein Female CD5 030217 neurology & neurosurgery |
| Zdroj: | Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology. 13(1) |
| ISSN: | 1557-1904 |
| Popis: | Pro-inflammatory activity and cell-mediated immune responses have been widely observed in patients with major depressive disorder (MDD). Besides their well-known function as antibody-producers, B cells play a key role in inflammatory responses by secreting pro- and anti-inflammatory factors. However, homeostasis of specific B cell subsets has not been comprehensively investigated in MDD. In this study, we characterized circulating B cells of distinct developmental steps including transitional, naive-mature, antigen-experienced switched, and non-switched memory cells, plasmablasts and regulatory B cells by multi-parameter flow cytometry. In a 6-weeks follow-up, circulating B cells were monitored in a small group of therapy responders and non-responders. Frequencies of naive lgD+CD27− B cells, but not lgD+CD27+ memory B cells, were reduced in severely depressed patients as compared to healthy donors (HD) or mildly to moderately depressed patients. Specifically, B cells with immune-regulatory capacities such as CD1d+CD5+ B cells and CD24+CD38hi transitional B cells were reduced in MDD. Also Bm1-Bm5 classification in MDD revealed reduced Bm2’ cells comprising germinal center founder cells as well as transitional B cells. We further found that reduced CD5 surface expression on transitional B cells was associated with severe depression and normalized exclusively in clinical responders. This study demonstrates a compromised peripheral B cell compartment in MDD with a reduction in B cells exhibiting a regulatory phenotype. Recovery of CD5 surface expression on transitional B cells in clinical response, a molecule involved in activation and down-regulation of B cell responses, further points towards a B cell-dependent process in the pathogenesis of MDD. |
| Databáze: | OpenAIRE |
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