The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features
| Autor: | Holger Brüggemann, Rudolf K. Thauer, Henning Seedorf, Birgit Veith, Fu-Li Li, Axel Strittmatter, W. Florian Fricke, Marcus Miethke, Christoph H. Hagemeier, Heiko Liesegang, Wolfgang Buckel, Julia Hinderberger, Gerhard Gottschalk |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Glycerol
Molecular Sequence Data Succinic Acid Acetates 7. Clean energy Clostridia 03 medical and health sciences Phenols Nonribosomal peptide Oxidoreductase Ferredoxin 030304 developmental biology Alcohol dehydrogenase chemistry.chemical_classification 0303 health sciences Multidisciplinary Butyryl-CoA dehydrogenase biology Ethanol 030306 microbiology Clostridium kluyveri Alcohol Dehydrogenase Biological Sciences biology.organism_classification Aldehyde Oxidoreductases Thiazoles chemistry Biochemistry Acetic Acids Fermentation biology.protein Energy source Genome Bacterial |
| Zdroj: | Seedorf, H, Fricke, W F, Veith, B, Brüggemann, H, Liesegang, H, Strittmatter, A, Miethke, M, Buckel, W, Hinderberger, J, Li, F, Hagemeier, C, Thauer, R K & Gottschalk, G 2008, ' The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features ' Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 6, pp. 2128-33 . https://doi.org/10.1073/pnas.0711093105 |
| DOI: | 10.1073/pnas.0711093105 |
| Popis: | Clostridium kluyveriis unique among the clostridia; it grows anaerobically on ethanol and acetate as sole energy sources. Fermentation products are butyrate, caproate, and H2. We report here the genome sequence ofC. kluyveri, which revealed new insights into the metabolic capabilities of this well studied organism. A membrane-bound energy-converting NADH:ferredoxin oxidoreductase (RnfCDGEAB) and a cytoplasmic butyryl-CoA dehydrogenase complex (Bcd/EtfAB) coupling the reduction of crotonyl-CoA to butyryl-CoA with the reduction of ferredoxin represent a new energy-conserving module in anaerobes. The genes for NAD-dependent ethanol dehydrogenase and NAD(P)-dependent acetaldehyde dehydrogenase are located next to genes for microcompartment proteins, suggesting that the two enzymes, which are isolated together in a macromolecular complex, form a carboxysome-like structure. Unique for a strict anaerobe,C. kluyveriharbors three sets of genes predicted to encode for polyketide/nonribosomal peptide synthetase hybrides and one set for a nonribosomal peptide synthetase. The latter is predicted to catalyze the synthesis of a new siderophore, which is formed under iron-deficient growth conditions. |
| Databáze: | OpenAIRE |
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