Comparative Genomic Analysis Confirms Five Genetic Populations of the Select Agent, Rathayibacter toxicus
| Autor: | James P. Stack, Rachel Mann, Grethel Y. Busot, Brendan Rodoni, Jarred Yasuhara-Bell, Mohammad Arif |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Comparative genomics Genetics 0303 health sciences Future studies annual ryegrass toxicity 030306 microbiology Select agent flood plain staggers comparative genomics Biology rathayibacter toxicus populations Microbiology Genome 03 medical and health sciences stuart’s range syndrome Rathayibacter toxicus lcsh:Biology (General) Genus Virology Comparative genomic analysis Gene lcsh:QH301-705.5 030304 developmental biology |
| Zdroj: | Microorganisms, Vol 8, Iss 3, p 366 (2020) Microorganisms Volume 8 Issue 3 |
| ISSN: | 2076-2607 |
| Popis: | Rathayibacter toxicus is a Gram-positive, nematode-vectored bacterium that infects several grass species in the family Poaceae. Unique in its genus, R. toxicus has the smallest genome, possesses a complete CRISPR-Cas system, a vancomycin-resistance cassette, produces tunicamycin, a corynetoxin responsible for livestock deaths in Australia, and is designated a Select Agent in the United States. In-depth, genome-wide analyses performed in this study support the previously designated five genetic populations, with a core genome comprising approximately 80% of the genome for all populations. Results varied as a function of the type of analysis and when using different bioinformatics tools for the same analysis e.g., some programs failed to identify specific genomic regions that were actually present. The software variance highlights the need to verify bioinformatics results by additional methods e.g., PCR, mapping genes to genomes, use of multiple algorithms). These analyses suggest the following relationships among populations: RT-IV &harr RT-I &harr RT-II &harr RT-III &harr RT-V, with RT-IV and RT-V being the most unrelated. This is the most comprehensive analysis of R. toxicus that included populations RT-I and RT-V. Future studies require underrepresented populations and more recent isolates from varied hosts and geographic locations. |
| Databáze: | OpenAIRE |
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